Production of hairy root cultures of lettuce (Lactuca sativa L.)

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Abstract: Hairy root cultures of lettuce (Lactuca sativa L.) were obtained by inoculation of cotyledonary leaves of in vitro lettuce seedlings (cvs. Nansen and Ljubljanska ledenka) with Agrobacterium rhizogenes A4M70GUS. Approximately in 96.7% cvs. Nansen and in 91.2% Ljubljanska ledenka inoculated explants produced hairy root when they were incubated on Murashige and Skoog (MS) half-strength medium without plant growth regulators. A total of 54% of all hairy root cultures expressed GUS activity. Every hairy root represented an independent transformation event. Line Ljubljanska ledenka 18 showed the highest biomass (5.5 times the biomass of control root). A PCR analysis of the genomic DNA confirmed the presence of marker and target genes in 15 hairy roots examined.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
Production of hairy root cultures of lettuce (Lactuca sativa L.) ; volume:9 ; number:12 ; year:2014 ; pages:1196-1205 ; extent:10
Open life sciences ; 9, Heft 12 (2014), 1196-1205 (gesamt 10)

Urheber
Vojin, Tadić
Snežana, Milošević
Aleksandar, Cingel
Marija, Petrić
Milana, Trifunović
Dragana, Antonić
Jovan, Tadić
Angelina, Subotić

DOI
10.2478/s11535-014-0351-9
URN
urn:nbn:de:101:1-2409211613087.031447466796
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:23 MESZ

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Beteiligte

  • Vojin, Tadić
  • Snežana, Milošević
  • Aleksandar, Cingel
  • Marija, Petrić
  • Milana, Trifunović
  • Dragana, Antonić
  • Jovan, Tadić
  • Angelina, Subotić

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