Label‐Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells
Abstract: Tumor cells present profound alterations in their composition, structural organization, and functional properties. A landmark of cancer cells is an overall altered mechanical phenotype, which so far are linked to changes in their cytoskeletal regulation and organization. Evidence exists that the plasma membrane (PM) of cancer cells also shows drastic changes in its composition and organization. However, biomechanical characterization of PM remains limited mainly due to the difficulties encountered to investigate it in a quantitative and label‐free manner. Here, the biomechanical properties of PM of a series of MCF10 cell lines, used as a model of breast cancer progression, are investigated. Notably, a strong correlation between the cell PM elasticity and oncogenesis is observed. The altered membrane composition under cancer progression, as emphasized by the PM‐associated cholesterol levels, leads to a stiffening of the PM that is uncoupled from the elastic cytoskeletal properties. Conversely, cholesterol depletion of metastatic cells leads to a softening of their PM, restoring biomechanical properties similar to benign cells. As novel therapies based on targeting membrane lipids in cancer cells represent a promising approach in the field of anticancer drug development, this method contributes to deciphering the functional link between PM lipid content and disease.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Label‐Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells ; volume:7 ; number:22 ; year:2020 ; extent:13
Advanced science ; 7, Heft 22 (2020) (gesamt 13)
- Urheber
-
Dumitru, Andra C.
Mohammed, Danahe
Maja, Mauriane
Yang, Jinsung
Verstraeten, Sandrine
Campo, Aránzazu del
Mingeot‐Leclercq, Marie‐Paule
Tyteca, Donatienne
Alsteens, David
- DOI
-
10.1002/advs.202002643
- URN
-
urn:nbn:de:101:1-2022052707201053260391
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:31 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Dumitru, Andra C.
- Mohammed, Danahe
- Maja, Mauriane
- Yang, Jinsung
- Verstraeten, Sandrine
- Campo, Aránzazu del
- Mingeot‐Leclercq, Marie‐Paule
- Tyteca, Donatienne
- Alsteens, David
Ähnliche Objekte (12)
Label-Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells
Polyacrylamide Hydrogels as Versatile Biomimetic Platforms to Study Cell‐Materials Interactions
Generating micro- and nanopatterns on polymeric materials
Functional and safe encapsulation of Escherichia coli in Pluronic hydrogels for engineered living materials
Light‐Regulated Angiogenesis via a Phototriggerable VEGF Peptidomimetic
Optoregulation of collagen biosynthesis and remodeling in collagen associated diseases
Microenvironments to regulate cellular behavior for neural development and regeneration
Photodeagradable hydrogels for tissue gluing
Two-photon sensitive biomaterials for dynamic control of cellular microenvironments
o-Nitrobenzyl-based polymer materials with light-regulated multi-functions
Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity
Tetrazole Methylsulfone - Thiol crosslinked hydrogels for automated and high-throughput 3D cell culture
Label-Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells
Polyacrylamide Hydrogels as Versatile Biomimetic Platforms to Study Cell‐Materials Interactions
Generating micro- and nanopatterns on polymeric materials
Functional and safe encapsulation of Escherichia coli in Pluronic hydrogels for engineered living materials
Light‐Regulated Angiogenesis via a Phototriggerable VEGF Peptidomimetic
Optoregulation of collagen biosynthesis and remodeling in collagen associated diseases
Microenvironments to regulate cellular behavior for neural development and regeneration
Photodeagradable hydrogels for tissue gluing
Two-photon sensitive biomaterials for dynamic control of cellular microenvironments
o-Nitrobenzyl-based polymer materials with light-regulated multi-functions
Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity
Tetrazole Methylsulfone - Thiol crosslinked hydrogels for automated and high-throughput 3D cell culture
Label-Free Imaging of Cholesterol Assemblies Reveals Hidden Nanomechanics of Breast Cancer Cells
Polyacrylamide Hydrogels as Versatile Biomimetic Platforms to Study Cell‐Materials Interactions
Generating micro- and nanopatterns on polymeric materials
Functional and safe encapsulation of Escherichia coli in Pluronic hydrogels for engineered living materials
Light‐Regulated Angiogenesis via a Phototriggerable VEGF Peptidomimetic
Optoregulation of collagen biosynthesis and remodeling in collagen associated diseases
Microenvironments to regulate cellular behavior for neural development and regeneration
Photodeagradable hydrogels for tissue gluing
Two-photon sensitive biomaterials for dynamic control of cellular microenvironments
o-Nitrobenzyl-based polymer materials with light-regulated multi-functions
Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity