Development of tularemic scFv antibody fragments using phage display
Abstract: Polyclonal antibodies, as well as monoclonal antibodies are efficacious in providing protective immunity against Francisella tularensis. This study demonstrates the application of phage display libraries for the construction of monoclonal antibodies against F. tularensis. Novel single-chain fragment variable (scFv) antibodies were generated against a whole bacterial lysate of F. tularensis live vaccine strain using the human single fold scFv libraries I (Tomlinson I + J). A total of 20 clones reacted with the bacterial cell lysate. Further, the library contains two clones responsive to recombinant lipoprotein FTT1103Δsignal (F. tularensis subsp. tularensis Schu S4), which was constructed without a signal sequence. These positively-binding scFvs were evaluated by scFv-phage enzyme-linked immunosorbent assay (ELISA). Then, positive scFvs were expressed in a soluble form in Escherichia coli HB2151 and tested for positive scFvs by using scFv-ELISA.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Development of tularemic scFv antibody fragments using phage display ; volume:5 ; number:3 ; year:2010 ; pages:310-317 ; extent:8
Open life sciences ; 5, Heft 3 (2010), 310-317 (gesamt 8)
- Creator
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Kubelkova, Klara
Macela, Ales
- DOI
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10.2478/s11535-010-0015-3
- URN
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urn:nbn:de:101:1-2409201854397.794686287233
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:34 AM CEST
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Associated
- Kubelkova, Klara
- Macela, Ales
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