Enzymatic glyco-modification of synthetic membrane systems
Abstract: The present report assesses the capability of a soluble glycosyltransferase to modify glycolipids organized in two synthetic membrane systems that are attractive models to mimic cell membranes: giant unilamellar vesicles (GUVs) and supported lipid bilayers (SLBs). The objective was to synthesize the Gb3 antigen (Galα1,4Galβ1,4Glcβ-Cer), a cancer biomarker, at the surface of these membrane models. A soluble form of LgtC that adds a galactose residue from UDP-Gal to lactose-containing acceptors was selected. Although less efficient than with lactose, the ability of LgtC to utilize lactosyl–ceramide as an acceptor was demonstrated on GUVs and SLBs. The reaction was monitored using the B-subunit of Shiga toxin as Gb3-binding lectin. Quartz crystal microbalance with dissipation analysis showed that transient binding of LgtC at the membrane surface was sufficient for a productive conversion of LacCer to Gb3. Molecular dynamics simulations provided structural elements to help rationalize experimental data
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Biomolecules. - 13, 2 (2023) , 335, ISSN: 2218-273X
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
-
Universität
- (wann)
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2023
- Urheber
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Jabeguero, Dylan
Siukstaite, Lina
Wang, Chunyue
Mitrovic, Anna
Perez, Serge
Makshakova, Olga N.
Richter, Ralf P.
Römer, Winfried
Breton, Christelle
- DOI
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10.3390/biom13020335
- URN
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urn:nbn:de:bsz:25-freidok-2336994
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:54 MEZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Jabeguero, Dylan
- Siukstaite, Lina
- Wang, Chunyue
- Mitrovic, Anna
- Perez, Serge
- Makshakova, Olga N.
- Richter, Ralf P.
- Römer, Winfried
- Breton, Christelle
- Universität
Entstanden
- 2023
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