Skin‐draining lymph node priming is sufficient to induce sterile immunity against pre‐erythrocytic malaria

Abstract: The Plasmodium‐infected hepatocyte has been considered necessary to prime the immune responses leading to sterile protection after vaccination with attenuated sporozoites. However, it has recently been demonstrated that priming also occurs in the skin. We wished to establish if sterile protection could be obtained in the absence of priming by infected hepatocytes. To this end, we developed a subcutaneous (s.c.) immunization protocol where few, possibly none, of the immunizing irradiated Plasmodium yoelii sporozoites infect hepatocytes, and also used CD81‐deficient mice non‐permissive to productive hepatocyte infections. We then compared and contrasted the patterns of priming with those obtained by intradermal immunization, where priming occurs in the liver. Using sterile immunity as a primary read‐out, we exploited an inhibitor of T‐cell migration, transgenic mice with conditional depletion of dendritic cells and adoptive transfers of draining lymph node‐derived T cells, to provide evidence that responses leading to sterile immunity can be primed in the skin‐draining lymph nodes with little, if any, contribution from the infected hepatocyte.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Skin‐draining lymph node priming is sufficient to induce sterile immunity against pre‐erythrocytic malaria ; volume:5 ; number:2 ; year:2013 ; pages:250-263 ; extent:14 ; EMBO molecular medicine / European Molecular Biology Organization ; 5, Heft 2 (2013), 250-263 (gesamt 14)

Contributors, Places and Time

• Creator: Obeid, Michel ; Franetich, Jean‐François ; Lorthiois, Audrey ; Gego, Audrey ; Grüner, Anne Charlotte ; Tefit, Maurel ; Boucheix, Claude ; Snounou, Georges ; Mazier, Dominique

Further information

• DOI: 10.1002/emmm.201201677
• URN: urn:nbn:de:101:1-2023020305494904008343
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:20 AM CEST