RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence

Abstract: Protein synthesis via ribosomes is a fundamental process in all known living organisms. However, it can be completely stalled by removing a single nucleobase (depurination) at the sarcin/ricin loop of the ribosomal RNA. In this work, we describe the preparation and optimization process of a fluorescent probe that can be used to visualize depurination. Starting from a fluorescent thiophene nucleobase analog, various RNA probes that fluoresce exclusively in the presence of a depurinated sarcin/ricin‐loop RNA were designed and characterized. The main challenge in this process was to obtain a high fluorescence signal in the hybridized state with an abasic RNA strand, while keeping the background fluorescence low. With our new RNA probes, the fluorescence intensity and lifetime can be used for efficient monitoring of depurinated RNA.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence ; day:18 ; month:11 ; year:2022 ; extent:1 ; Chemistry ; (18.11.2022) (gesamt 1)

Beteiligte, Orts- und Zeitangaben

• Urheber: Klimek, Robin ; Kaiser, Christoph ; Murmann, Nina S. ; Kaltenschnee, Nina ; Spanò, Teresa ; Wachtveitl, Josef ; Schuman, Erin M. ; Heckel, Alexander

Weitere Informationen

• DOI: 10.1002/asia.202201077
• URN: urn:nbn:de:101:1-2022111814324158885662
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:34 MESZ