Inhalable Dry Powders for Lung mRNA Delivery
Abstract: Despite great promise, application of mRNA therapeutics in the lung has proven challenging. Many groups have reported success instilling liquid mRNA formulations in animal models, but direct intratracheal administration of large liquid quantities to the human lung presents significant safety and distribution concerns. To accomplish safe and effective mRNA delivery to the lung, formulations must be prepared for dosing via inhalation. An inhaled mRNA delivery system for the lung must be both robust enough to survive inhalation conditions and potent enough to deliver mRNA upon reaching the lung. In this work dry powder lipid nanoparticle formulations are developed, using spray‐freeze‐drying, to produce stable, biologically active, inhalable dry powders for mRNA delivery. The final powders have suitable aerosolization properties, with mean mass aerodynamic diameter (MMAD) of 3–4 microns, and fine particle fraction (FPF) ≈40%, allowing for efficient mRNA delivery to the deep lung following inhalation. Importantly, the formulations developed here are suitable for use with different ionizable lipids. Four different ionizable lipid‐based formulations are evaluated as powders, and all exhibit in vivo pulmonary mRNA delivery equal to that of instilled liquid formulations. These results lay promising groundwork for the eventual development of an inhalable mRNA dry powder therapeutic.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Inhalable Dry Powders for Lung mRNA Delivery ; day:01 ; month:10 ; year:2024 ; extent:9
Advanced healthcare materials ; (01.10.2024) (gesamt 9)
- Urheber
-
Gordon, Akiva
Li, Bowen
Witten, Jacob
Nguyen, Hong
Anderson, Daniel G.
- DOI
-
10.1002/adhm.202400509
- URN
-
urn:nbn:de:101:1-2410021404331.406898211931
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:28 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Gordon, Akiva
- Li, Bowen
- Witten, Jacob
- Nguyen, Hong
- Anderson, Daniel G.
Ähnliche Objekte (12)
An Acid‐Controlled Method for the Regioselective Functionalization of Anilines over Aliphatic Amines
Diffusion in garnet: a review
Comparison of the therapeutic effect of Meibomian Thermal Pulsation LipiFlow® on obstructive and hyposecretory meibomian gland dysfunction patients
Comparison of condylar position after free fibular flap mandibular reconstruction using computer-assisted and traditional techniques
Research on panic spread and decision behaviour in a delayed SEIR evolutionary game model under an emergency
Composition optimization, high-temperature stability, and thermal cycling performance of Sc-doped Gd2Zr2O7 thermal barrier coatings: Theoretical and experimental studies
C–H Bond Functionalization of Amines: A Graphical Overview of Diverse Methods
AGILE platform: a deep learning powered approach to accelerate LNP development for mRNA delivery
Measuring tree stem diameters and straightness with depth-image computer vision
Acute Myeloid Leukemia with Myelodysplasia-Related Changes in a Patient with Crohn’s Disease Treated with Immunosuppressive Therapy
Effect of resistance vs. aerobic exercise in pre-diabetes: an RCT
Cold and hot tumors: from molecular mechanisms to targeted therapy
An Acid‐Controlled Method for the Regioselective Functionalization of Anilines over Aliphatic Amines
Diffusion in garnet: a review
Comparison of the therapeutic effect of Meibomian Thermal Pulsation LipiFlow® on obstructive and hyposecretory meibomian gland dysfunction patients
Comparison of condylar position after free fibular flap mandibular reconstruction using computer-assisted and traditional techniques
Research on panic spread and decision behaviour in a delayed SEIR evolutionary game model under an emergency
Composition optimization, high-temperature stability, and thermal cycling performance of Sc-doped Gd2Zr2O7 thermal barrier coatings: Theoretical and experimental studies
C–H Bond Functionalization of Amines: A Graphical Overview of Diverse Methods
AGILE platform: a deep learning powered approach to accelerate LNP development for mRNA delivery
Measuring tree stem diameters and straightness with depth-image computer vision
Acute Myeloid Leukemia with Myelodysplasia-Related Changes in a Patient with Crohn’s Disease Treated with Immunosuppressive Therapy
Effect of resistance vs. aerobic exercise in pre-diabetes: an RCT
Cold and hot tumors: from molecular mechanisms to targeted therapy
An Acid‐Controlled Method for the Regioselective Functionalization of Anilines over Aliphatic Amines
Diffusion in garnet: a review
Comparison of the therapeutic effect of Meibomian Thermal Pulsation LipiFlow® on obstructive and hyposecretory meibomian gland dysfunction patients
Comparison of condylar position after free fibular flap mandibular reconstruction using computer-assisted and traditional techniques
Research on panic spread and decision behaviour in a delayed SEIR evolutionary game model under an emergency
Composition optimization, high-temperature stability, and thermal cycling performance of Sc-doped Gd2Zr2O7 thermal barrier coatings: Theoretical and experimental studies
C–H Bond Functionalization of Amines: A Graphical Overview of Diverse Methods
AGILE platform: a deep learning powered approach to accelerate LNP development for mRNA delivery
Measuring tree stem diameters and straightness with depth-image computer vision
Acute Myeloid Leukemia with Myelodysplasia-Related Changes in a Patient with Crohn’s Disease Treated with Immunosuppressive Therapy
Effect of resistance vs. aerobic exercise in pre-diabetes: an RCT