A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. brucei

to related objects

Abstract: The AAA-ATPase Msp1 extracts mislocalised outer membrane proteins and thus contributes to mitochondrial proteostasis. Using pulldown experiments, we show that trypanosomal Msp1 localises to both glycosomes and the mitochondrial outer membrane, where it forms a complex with four outer membrane proteins. The trypanosome-specific pATOM36 mediates complex assembly of α-helically anchored mitochondrial outer membrane proteins such as protein translocase subunits. Inhibition of their assembly triggers a pathway that results in the proteasomal digestion of unassembled substrates. Using inducible single, double, and triple RNAi cell lines combined with proteomic analyses, we demonstrate that not only Msp1 but also the trypanosomal homolog of the AAA-ATPase VCP are implicated in this quality control pathway. Moreover, in the absence of VCP three out of the four Msp1-interacting mitochondrial proteins are required for efficient proteasomal digestion of pATOM36 substrates, suggesting they act in concert with Msp1. pATOM36 is a functional analog of the yeast mitochondrial import complex complex and possibly of human mitochondrial animal-specific carrier homolog 2, suggesting that similar mitochondrial quality control pathways linked to Msp1 might also exist in yeast and humans

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Notes: Life science alliance. - 6, 11 (2023) , e202302004, ISSN: 2575-1077

Event: Veröffentlichung

(where): Freiburg

(who): Universität

(when): 2023

Creator: Gerber, Markus
Suppanz, Ida
Oeljeklaus, Silke
Niemann, Moritz
Käser, Sandro
Warscheid, Bettina
Schneider, André
Dewar, Caroline E.

DOI: 10.26508/lsa.202302004

URN: urn:nbn:de:bsz:25-freidok-2386332

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 10:44 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Gerber, Markus
Suppanz, Ida
Oeljeklaus, Silke
Niemann, Moritz
Käser, Sandro
Warscheid, Bettina
Schneider, André
Dewar, Caroline E.
Universität

Time of origin

2023

Other Objects (12)

Hochschulschrift

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Hochschulschrift | Online-Publikation

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

$Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$$

Hochschulschrift

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$

Hochschulschrift

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Hochschulschrift | Online-Publikation

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

$Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$$

Hochschulschrift

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$

Hochschulschrift

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Hochschulschrift | Online-Publikation

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

$Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$$

Hochschulschrift

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in $\textit Saccharomyces cerevisiae}$

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.

A Msp1-containing complex removes orphaned proteins in the mitochondrial outer membrane of T. brucei

Object Details

Contributors, Places and Time

Further information

Data provider

Associated

Time of origin

Other Objects (12)

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in \(\textit Saccharomyces cerevisiae}\)

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in \(\textit Saccharomyces cerevisiae}\)

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Biogenesis of the mitochondrial DNA inheritance machinery in the mitochondrial outer membrane of Trypanosoma brucei

Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics

The endoplasmic reticulum membrane protein complex localizes to the mitochondrial - endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei

Determinism and contingencies shaped the evolution of mitochondrial protein import

A trypanosomal orthologue of an intermembrane space chaperone has a non-canonical function in biogenesis of the single mitochondrial inner membrane protein translocase

Identification of new channels by systematic analysis of the mitochondrial outer membrane

The Mba1 homologue of Trypanosoma brucei is involved in the biogenesis of oxidative phosphorylation complexes

Order from disorder in the sarcomere: FATZ forms a fuzzy but tight complex and phase-separated condensates with α-actinin

Untersuchungen zur Biogenese von Peroxisomen : proteomische Charakterisierung der Interaktome von Pex14p und Pex3p in \(\textit Saccharomyces cerevisiae}\)

Related objects

Reset password