Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis
Abstract: Multiple studies have indicated that long non-coding RNAs are aberrantly expressed in cancers and are pivotal in developing various tumors. No studies have investigated the expression and function of long non-coding antisense RNA PCNA-AS1 in esophageal squamous cell carcinoma (ESCC). In this study, the expression of PCNA-AS1 was identified by qRT–PCR. Cell function assays were used to explore the potential effect of PCNA-AS1 on ESCC progression. A prediction website was utilized to discover the relationships among PCNA-AS1, miR-2467-3p and proliferating cell nuclear antigen (PCNA). Dual luciferase reporter gene and RNA immunoprecipitation (RIP) assays were executed to verify the binding activity between PCNA-AS1, miR-2467-3p and PCNA. As a result, PCNA-AS1 was highly expressed in ESCC and was associated with patient prognosis. PCNA-AS1 overexpression strongly contributed to ESCC cell proliferation, invasion and migration. PCNA-AS1 and PCNA were positively correlated in ESCC. Bioinformatics analysis, RIP and luciferase reporter gene assays revealed that PCNA-AS1 could act as a competitive endogenous RNA to sponge miR-2467-3p, thus upregulating PCNA. In conclusion, the current outcome demonstrates that PCNA-AS1 may be a star molecule in the treatment of ESCC.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Antisense lncRNA PCNA-AS1 promotes esophageal squamous cell carcinoma progression through the miR-2467-3p/PCNA axis ; volume:17 ; number:1 ; year:2022 ; pages:1483-1494 ; extent:12
Open medicine ; 17, Heft 1 (2022), 1483-1494 (gesamt 12)
- Creator
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Hu, Tao
Niu, Yunfeng
Fu, Jianfeng
Dong, Zhiming
He, Dongwei
Liu, Junfeng
- DOI
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10.1515/med-2022-0552
- URN
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urn:nbn:de:101:1-2022092114050123293737
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:35 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Hu, Tao
- Niu, Yunfeng
- Fu, Jianfeng
- Dong, Zhiming
- He, Dongwei
- Liu, Junfeng
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Immunhistometrische Analyse der Proliferationskinetik des Mammakarzinoms mit PCNA
Prognostic significance of PCNA grade in malignant melanoma
Funktionen und Regulation der PCNA-Ubiquitinierung in Vertebraten
Expression von PCNA (Zyklin) und Ki-67 in Schilddrüsengewebe
Bestimmung der Proliferationsmarker PCNA und Ki-67 beim Ovarialkarzinom
Regulation of replication-linked functions by PCNA and SUMO
Discrimination of cell cycle phases in PCNA-immunolabeled cells
ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair
Structures of the human leading strand Polε–PCNA holoenzyme
Bayesian estimation of equipment reliability with normal-type life distribution based on multiple batch tests
Readers of PCNA modifications
EZH2 promotes DNA replication by stabilizing interaction of POLδ and PCNA via methylation-mediated PCNA trimerization
Immunhistometrische Analyse der Proliferationskinetik des Mammakarzinoms mit PCNA
Prognostic significance of PCNA grade in malignant melanoma
Funktionen und Regulation der PCNA-Ubiquitinierung in Vertebraten
Expression von PCNA (Zyklin) und Ki-67 in Schilddrüsengewebe
Bestimmung der Proliferationsmarker PCNA und Ki-67 beim Ovarialkarzinom
Regulation of replication-linked functions by PCNA and SUMO
Discrimination of cell cycle phases in PCNA-immunolabeled cells
ATAD5-BAZ1B interaction modulates PCNA ubiquitination during DNA repair
Structures of the human leading strand Polε–PCNA holoenzyme