Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions
Abstract: A highly efficient cap‐exchange approach for preparing compact, dense polyvalent mannose‐capped quantum dots (QDs) has been developed. The resulting QDs have been successfully used to probe multivalent interactions of HIV/Ebola receptors DC‐SIGN and DC‐SIGNR (collectively termed as DC‐SIGN/R) using a sensitive, ratiometric Förster resonance energy transfer (FRET) assay. The QD probes specifically bind DC‐SIGN, but not its closely related receptor DC‐SIGNR, which is further confirmed by its specific blocking of DC‐SIGN engagement with the Ebola virus glycoprotein. Tuning the QD surface mannose valency reveals that DC‐SIGN binds more efficiently to densely packed mannosides. A FRET‐based thermodynamic study reveals that the binding is enthalpy‐driven. This work establishes QD FRET as a rapid, sensitive technique for probing structure and thermodynamics of multivalent protein–ligand interactions.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Compact, Polyvalent Mannose Quantum Dots as Sensitive, Ratiometric FRET Probes for Multivalent Protein–Ligand Interactions ; volume:128 ; number:15 ; year:2016 ; pages:4816-4820 ; extent:5
Angewandte Chemie ; 128, Heft 15 (2016), 4816-4820 (gesamt 5)
- Urheber
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Guo, Yuan
Sakonsinsiri, Chadamas
Nehlmeier, Inga
Fascione, Martin A.
Zhang, Haiyan
Wang, Weili
Pöhlmann, Stefan
Turnbull, W. Bruce
Zhou, Dejian
- DOI
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10.1002/ange.201600593
- URN
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urn:nbn:de:101:1-2022101006374820209155
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:26 MESZ
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Beteiligte
- Guo, Yuan
- Sakonsinsiri, Chadamas
- Nehlmeier, Inga
- Fascione, Martin A.
- Zhang, Haiyan
- Wang, Weili
- Pöhlmann, Stefan
- Turnbull, W. Bruce
- Zhou, Dejian
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