An optimized filter trap assay for detecting recombinant authentic tau fibrils
Abstract: The development and optimization of the Filter Trap Assay (FTA) for the detection of authentic tau fibrils in vitro mark a pivotal advancement in the realm of tauopathy research, particularly by addressing the limitations of using polyanion-induced tau fibrils, which structurally differ from those isolated from tauopathy patients. Recently it has been shown that truncated tau fragment (297-391), also termed dGAE, can form authentic tau fibrils in the absence of polyanions. This study introduces a refined protocol that reliably detects authentic tau fibrils in a physiologically relevant framework, utilizing nitrocellulose membranes to achieve heightened sensitivity. Our investigation highlights the superior efficacy of sarkosyl, an anionic surfactant traditionally used to prepare protein lysates from brains and cultured neurons, in preserving the aggregated state of tau dGAE fibrils in vitro, underscoring its potential for further exploratory studies. By offering a user-friendly and.... https://www.uni-muenster.de/Ejournals/index.php/fnp/article/view/5497
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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An optimized filter trap assay for detecting recombinant authentic tau fibrils ; volume:5 ; year:2024
Free Neuropathology ; 5 (2024)
- Urheber
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Balaj, Allison R.
Rothstein, Thomas L.
Kaku, Hiroaki
- DOI
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10.17879/freeneuropathology-2024-5497
- URN
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urn:nbn:de:hbz:6:3-freeneuropathology-2024-59015
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:21 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Balaj, Allison R.
- Rothstein, Thomas L.
- Kaku, Hiroaki
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