Dexmedetomidine may decrease the bupivacaine toxicity to heart
Objective: The purpose of our study was to explore the effect of dexmedetomidine on cardiac tolerance to bupivacaine. Method: Human coronary endothelial cells were used to establish in vitro model. They were randomly divided into control (Con) group, dexmedetomidine (Dex) group, bupivacaine (Bupi) group, dexmedetomidine + bupivacaine group (DB group), and dexmedetomidine + bupivacaine + PI3K inhibitor (DB-inhibitor) group. Cell activity was measured by Cell counting kit-8 (CCK-8). Transwell was used to detect cell permeability. Western blotting was used to detect the protein expression of related factors. Results: There were no notable differences in cell activity among the five groups (P > 0.05). Dexmedetomidine significantly reduced the permeability of endothelial cells to bupivacaine and increased the protein expression of Zonulaoeeludens-1 (ZO-1) (P < 0.01). However, the aforementioned effects of dexmedetomidine were disappeared after the addition of PI3K inhibitors. Furthermore, Dex and DB markedly increased the protein expression of PI3K, p-Akt, and p-PTEN in comparison with Con group (P < 0.001), but there was no significant difference in p-PTEN among DB-inhibitor, Con, and Bupi groups (P > 0.05). Conclusion: Dex reduced Bupi-induced vasopermeability through protein expression of ZO-1 and PI3K/Akt pathway, which may lead to the decrease of Bupi-induced cardiotoxicity.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Dexmedetomidine may decrease the bupivacaine toxicity to heart ; volume:16 ; number:1 ; year:2021 ; pages:1070-1075 ; extent:6
Open medicine ; 16, Heft 1 (2021), 1070-1075 (gesamt 6)
- Creator
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Jin, Zhousheng
Xia, Fangfang
Lin, Tingting
Cai, Yaoyao
Chen, Hongfei
Wang, Yuelan
- DOI
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10.1515/med-2021-0311
- URN
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urn:nbn:de:101:1-2022092014070342442297
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:38 AM CEST
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Jin, Zhousheng
- Xia, Fangfang
- Lin, Tingting
- Cai, Yaoyao
- Chen, Hongfei
- Wang, Yuelan
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Intrathecal bupivacaine with clonidine or dexmedetomidine as adjuvant in gynecological surgery: an enigma
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