Evaluation of Dibenzocyclooctyne and Bicyclononyne Click Reaction on Azido‐Functionalized Antifouling Polymer Brushes via Microspotting
Abstract: Strain‐promoted alkyne‐azide cycloaddition (SPAAC) has become an indispensable tool in bioorthogonal conjugation and surface immobilization. While numerous studies have focused on enhancing the reactivity of cyclooctynes, a facile method to evaluate the binding efficiency for cyclooctyne‐azide‐based immobilization without any sophisticated facilities is still missing. In the present work, different derivatives of dibenzocyclooctyne/bicyclononyne (DBCO/BCN) linked to either a fluorophore or a biotin‐moiety are patterned on ultra‐low fouling polymer brushes, which can avoid unspecific protein contamination without any prior blocking steps. The polymer brushes are composed of an antifouling bottom block and azide‐terminated top block. The assessment of binding efficiency is conducted on ordered arrays spotted by microchannel cantilever spotting (μCS) with a normal fluorescent microscope. Both cyclooctynes demonstrate reliable binding performance with azide‐bearing diblock polymer brushes via μCS, but DBCO shows a higher surface density of molecular immobilization according to the protein binding assays. This work provides a reference for choosing appropriate cyclooctyne to couple with azides and can be useful for the design of biosensors or bio‐platforms for analyte detection, cell capture, and other biological applications.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Evaluation of Dibenzocyclooctyne and Bicyclononyne Click Reaction on Azido‐Functionalized Antifouling Polymer Brushes via Microspotting ; day:14 ; month:04 ; year:2022 ; extent:10
Advanced materials interfaces ; (14.04.2022) (gesamt 10)
- Creator
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Yang, Bingquan
Wang, Yangxin
Vorobii, Mariia
Sauter, Eric
König, Meike
Kumar, Ravi
Rodriguez-Emmenegger, Cesar
Hirtz, Michael Manfred
- DOI
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10.1002/admi.202102325
- URN
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urn:nbn:de:101:1-2022041415215272888837
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:37 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Yang, Bingquan
- Wang, Yangxin
- Vorobii, Mariia
- Sauter, Eric
- König, Meike
- Kumar, Ravi
- Rodriguez-Emmenegger, Cesar
- Hirtz, Michael Manfred
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Thermoresponsive, Pyrrolidone‐Based Antifouling Polymer Brushes
Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria
Controlled Surface Adhesion of Macrophages via Patterned Antifouling Polymer Brushes
Controlled Surface Adhesion of Macrophages via Patterned Antifouling Polymer Brushes
Molecular Design of Antifouling Polymer Brushes Using Sequence‐Specific Peptoids
Salt‐Responsive Switchable Block Copolymer Brushes with Antibacterial and Antifouling Properties
Polymer Brush-Functionalized Chitosan Hydrogels as Antifouling Implant Coatings
Flexible and Biocompatible Antifouling Polyurethane Surfaces Incorporating Tethered Antimicrobial Peptides through Click Reactions
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Direct imaging of glycans in Arabidopsis roots via click labeling of metabolically incorporated azido-monosaccharides
Über Azido-Metallkomplexe
Evaluation of Dibenzocyclooctyne and Bicyclononyne Click Reaction on Azido-Functionalized Antifouling Polymer Brushes via Microspotting
Thermoresponsive, Pyrrolidone‐Based Antifouling Polymer Brushes
Click-correlative light and electron microscopy (click-AT-CLEM) for imaging and tracking azido-functionalized sphingolipids in bacteria
Controlled Surface Adhesion of Macrophages via Patterned Antifouling Polymer Brushes
Controlled Surface Adhesion of Macrophages via Patterned Antifouling Polymer Brushes
Molecular Design of Antifouling Polymer Brushes Using Sequence‐Specific Peptoids
Salt‐Responsive Switchable Block Copolymer Brushes with Antibacterial and Antifouling Properties
Polymer Brush-Functionalized Chitosan Hydrogels as Antifouling Implant Coatings
Flexible and Biocompatible Antifouling Polyurethane Surfaces Incorporating Tethered Antimicrobial Peptides through Click Reactions
“Clickable” and Antifouling Block Copolymer Brushes as a Versatile Platform for Peptide‐Specific Cell Attachment
Direct imaging of glycans in Arabidopsis roots via click labeling of metabolically incorporated azido-monosaccharides