resPAINT: Accelerating Volumetric Super‐Resolution Localisation Microscopy by Active Control of Probe Emission **

Abstract: Points for accumulation in nanoscale topography (PAINT) allows practically unlimited measurements in localisation microscopy but is limited by background fluorescence at high probe concentrations, especially in volumetric imaging. We present reservoir‐PAINT (resPAINT), which combines PAINT and active control of probe photophysics. In resPAINT, an activatable probe “reservoir” accumulates on target, enabling a 50‐fold increase in localisation rate versus conventional PAINT, without compromising contrast. By combining resPAINT with large depth‐of‐field microscopy, we demonstrate super‐resolution imaging of entire cell surfaces. We generalise the approach by implementing various switching strategies and 3D imaging techniques. Finally, we use resPAINT with a Fab to image membrane proteins, extending the operating regime of PAINT to include a wider range of biological interactions.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: resPAINT: Accelerating Volumetric Super‐Resolution Localisation Microscopy by Active Control of Probe Emission ** ; day:23 ; month:08 ; year:2022 ; extent:1 ; Angewandte Chemie / International edition. International edition ; (23.08.2022) (gesamt 1)

Contributors, Places and Time

• Creator: Sanders, Edward W. ; Carr, Alexander R. ; Bruggeman, Ezra ; Körbel, Markus ; Benaissa, Sarah I. ; Donat, Robert F. ; Santos, Ana M. ; McColl, James ; O'Holleran, Kevin ; Klenerman, David ; Davis, Simon J. ; Lee, Steven F. ; Ponjavic, Aleks

Further information

• DOI: 10.1002/anie.202206919
• URN: urn:nbn:de:101:1-2022082415084593051919
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:35 AM CEST