A Tumor Microenvironment Model of Pancreatic Cancer to Elucidate Responses toward Immunotherapy
Abstract: Pancreatic cancer is a devastating malignancy with minimal treatment options. Standard‐of‐care therapy, including surgery and chemotherapy, is unsatisfactory, and therapies harnessing the immune system have been unsuccessful in clinical trials. Resistance to therapy and disease progression are mediated by the tumor microenvironment, which contains excessive amounts of extracellular matrix and stromal cells, acting as a barrier to drug delivery. There is a lack of preclinical pancreatic cancer models that reconstruct the extracellular, cellular, and biomechanical elements of tumor tissues to assess responses toward immunotherapy. To address this limitation and explore the effects of immunotherapy in combination with chemotherapy, a multicellular 3D cancer model using a star‐shaped poly (ethylene glycol)–heparin hydrogel matrix is developed. Human pancreatic cancer cells, cancer‐associated fibroblasts, and myeloid cells are grown encapsulated in hydrogels to mimic key components of tumor tissues, and cell responses toward treatment are assessed. Combining the CD11b agonist ADH‐503 with anti‐PD‐1 immunotherapy and chemotherapy leads to a significant reduction in tumor cell viability, proliferation, metabolic activity, immunomodulation, and secretion of immunosuppressive and tumor growth‐promoting cytokines.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
A Tumor Microenvironment Model of Pancreatic Cancer to Elucidate Responses toward Immunotherapy ; day:11 ; month:12 ; year:2022 ; extent:15
Advanced healthcare materials ; (11.12.2022) (gesamt 15)
- Urheber
-
Kast, Verena
Nadernezhad, Ali
Pette, Dagmar
Gabrielyan, Anastasiia
Fusenig, Maximilian
Honselmann, Kim C.
Stange, Daniel E.
Werner, Carsten
Loessner, Daniela
- DOI
-
10.1002/adhm.202201907
- URN
-
urn:nbn:de:101:1-2022121214115232723685
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:20 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Kast, Verena
- Nadernezhad, Ali
- Pette, Dagmar
- Gabrielyan, Anastasiia
- Fusenig, Maximilian
- Honselmann, Kim C.
- Stange, Daniel E.
- Werner, Carsten
- Loessner, Daniela
Ähnliche Objekte (12)
Der Auslandsbereich der Parteistiftungen der Bundesrepublik Deutschland
12 years more than just skin and bones: the CRC Transregio 67
Polymers for regenerative medicine
Blood compatible membranes through surface functionalization
Development of an in vitro blood flow model to evaluate antimicrobial coatings for blood-contacting devices
Chemokine‐Capturing Wound Contact Layer Rescues Dermal Healing
Entwicklung von Verfahren zur Erzeugung anisotroper Mikrostrukturen und VEGF-Gradienten auf Collagen Typ I-Scaffolds für Zellkulturanwendungen
Engineering the transport of signaling molecules in glycosaminoglycan-based hydrogels
Targeting the leukemic stem cell niche : An opportunity for novel therapeutic treatment options
EMT‐Induced Cell‐Mechanical Changes Enhance Mitotic Rounding Strength
The springtail cuticle as a blueprint for omniphobic surfaces
Inkjet bioprinting and 3D culture of human MSC-laden binary starPEG-heparin hydrogels for cartilage tissue engineering
Der Auslandsbereich der Parteistiftungen der Bundesrepublik Deutschland
12 years more than just skin and bones: the CRC Transregio 67
Polymers for regenerative medicine
Blood compatible membranes through surface functionalization
Development of an in vitro blood flow model to evaluate antimicrobial coatings for blood-contacting devices
Chemokine‐Capturing Wound Contact Layer Rescues Dermal Healing
Entwicklung von Verfahren zur Erzeugung anisotroper Mikrostrukturen und VEGF-Gradienten auf Collagen Typ I-Scaffolds für Zellkulturanwendungen
Engineering the transport of signaling molecules in glycosaminoglycan-based hydrogels
Targeting the leukemic stem cell niche : An opportunity for novel therapeutic treatment options
EMT‐Induced Cell‐Mechanical Changes Enhance Mitotic Rounding Strength
The springtail cuticle as a blueprint for omniphobic surfaces
Inkjet bioprinting and 3D culture of human MSC-laden binary starPEG-heparin hydrogels for cartilage tissue engineering
Der Auslandsbereich der Parteistiftungen der Bundesrepublik Deutschland
12 years more than just skin and bones: the CRC Transregio 67
Polymers for regenerative medicine
Blood compatible membranes through surface functionalization
Development of an in vitro blood flow model to evaluate antimicrobial coatings for blood-contacting devices
Chemokine‐Capturing Wound Contact Layer Rescues Dermal Healing
Entwicklung von Verfahren zur Erzeugung anisotroper Mikrostrukturen und VEGF-Gradienten auf Collagen Typ I-Scaffolds für Zellkulturanwendungen
Engineering the transport of signaling molecules in glycosaminoglycan-based hydrogels
Targeting the leukemic stem cell niche : An opportunity for novel therapeutic treatment options
EMT‐Induced Cell‐Mechanical Changes Enhance Mitotic Rounding Strength
The springtail cuticle as a blueprint for omniphobic surfaces