Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells

Abstract: This research examines the cytotoxic consequences of thymoquinone-loaded bovine serum albumin nanoparticles (TQ-BSA NPs) on the A549 lung cancer cell line. UV-visible (UV–Vis) spectroscopy, Fourier transform infrared spectrophotometer (FT-IR), scanning electron microscopy (SEM), and dynamic light scattering (DLS) were employed to verify the biogenic TQ-BSA NPs’ size, shape, and distribution. UV–Vis spectrophotometry indicated peaks at 200–300 nm, 500–600 nm, and a prominent peak at 700–800 nm, confirming the presence of TQ-BSA NPs. The polydispersity index, as confirmed by DLS, indicated a solvent distribution in water, accompanied by a zeta potential value of 126.2 ± 46.8 mV. The average size of TQ-BSA NPs was confirmed to be 187 ± 8 nm by SEM. TQ-BSA NPs reduce colony formation in the A549 lung cancer cell line in a dose-dependent manner relative to the control group. Protein expression analysis indicated that TQ-BSA NPs promoted programmed cell death by increasing pro-apoptotic levels and decreasing anti-apoptotic levels. TQ-BSA NPs demonstrated inhibition of cancer cell proliferation and promotion of apoptosis and exhibited significant efficacy against cancer cells at low concentrations. As a result, they have the makings of a promising chemotherapeutic agent for low-dose, long-term administration.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Unleashing of cytotoxic effects of thymoquinone-bovine serum albumin nanoparticles on A549 lung cancer cells ; volume:19 ; number:1 ; year:2024 ; extent:15 ; Open life sciences ; 19, Heft 1 (2024) (gesamt 15)

Contributors, Places and Time

• Creator: Durga, Bala Baskaran ; Ramachandran, Vinayagam ; Senthil, Bakthavatchalam ; Soloman, Vasthi Gnanarani ; Elshikh, Mohamed Soliman ; Almutairi, Saeedah Musaed ; Wen, Zhi-Hong ; Lo, Yi-Hao

Further information

• DOI: 10.1515/biol-2022-1000
• URN: urn:nbn:de:101:1-2411281822102.075981577080
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:38 AM CEST