Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets
Abstract: The Min proteins from E.coli position the bacterial cell‐division machinery through pole‐to‐pole oscillations. In vitro, Min protein self‐organization can be reconstituted in the presence of a lipid membrane as a catalytic surface. However, Min dynamics have so far not been reconstituted in fully membrane‐enclosed volumes. Microdroplets interfaced by lipid monolayers were employed as a simple 3D mimic of cellular compartments to reconstitute Min protein oscillations. We demonstrate that lipid monolayers are sufficient to fulfil the catalytic role of the membrane and thus represent a facile platform to investigate Min protein regulated dynamics of the cell‐division protein FtsZ‐mts. In particular, we show that droplet containers reveal distinct Min oscillation modes, and reveal a dependence of FtsZ‐mts structures on compartment size. Finally, co‐reconstitution of Min proteins and FtsZ‐mts in droplets yields antagonistic localization, thus demonstrating that droplets indeed support the analysis of complex bacterial self‐organization in confined volumes.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets ; volume:55 ; number:43 ; year:2016 ; pages:13455-13459 ; extent:5
Angewandte Chemie / International edition. International edition ; 55, Heft 43 (2016), 13455-13459 (gesamt 5)
- Creator
- DOI
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10.1002/anie.201606069
- URN
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urn:nbn:de:101:1-2022103106523026368268
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:28 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Zieske, Katja
- Chwastek, Grzegorz
- Schwille, Petra
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Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets
Wave propagation in lipid monolayers
Lipid monolayers coupled to polyelectrolyte multilayers : stability, dynamics and interactions
Interactions of cationic peptides with anionic lipid bilayers and monolayers : influence of peptide and lipid modifications on binding
Characterization of proteins with intrinsic disorder in solution and in lipid monolayers
Method for the Monte Carlo based simulation of lipid-monolayers including lipid movement
Zwitterionic Character and Lipid Composition Determine the Behaviour of Glycosylphosphatidylinositol Fragments in Monolayers
Characterisation of the effects of intrinsically disordered protein (IDP)-solvent and IDP-lipid interactions in aqueous solution and lipid monolayers
Simultaneously propagating voltage and pressure pulses in lipid monolayers of pork brain and synthetic lipids
Propagation of 2D pressure pulses in lipid monolayers and its possible implications for biology
Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins
Micelles, membranes, microemulsions, and monolayers
Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets
Wave propagation in lipid monolayers
Lipid monolayers coupled to polyelectrolyte multilayers : stability, dynamics and interactions
Interactions of cationic peptides with anionic lipid bilayers and monolayers : influence of peptide and lipid modifications on binding
Characterization of proteins with intrinsic disorder in solution and in lipid monolayers
Method for the Monte Carlo based simulation of lipid-monolayers including lipid movement
Zwitterionic Character and Lipid Composition Determine the Behaviour of Glycosylphosphatidylinositol Fragments in Monolayers
Characterisation of the effects of intrinsically disordered protein (IDP)-solvent and IDP-lipid interactions in aqueous solution and lipid monolayers
Simultaneously propagating voltage and pressure pulses in lipid monolayers of pork brain and synthetic lipids
Propagation of 2D pressure pulses in lipid monolayers and its possible implications for biology
Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins