5-ALA-Induced Fluorescent Urine Cytology in Comparison with Conventional Cytology, BTA-TRAK, and NMP-22 Tests in the Diagnosis of Bladder Cancer

Abstract: Introduction To assess the sensitivity fluorescent urine cytology induced by 5-aminolevulinic acid (5-ALA) in the diagnosis of bladder cancer and to compare the sensitivity and specificity with currently available markers approved by the United States Food and Drug Administration (FDA), bladder tumour antigen (BTA-TRAK, Bard Diagnostic Sciences, Redmond, WA, United States) assay based on enzyme-linked immunosorbent assay (ELISA), nuclear matrix protein 22 (NMP-22), and conventional cytology. Materials and Methods Age- and gender-matched patients ≥ 18 years of age admitted with imaging-confirmed bladder cancer and non-malignant conditions formed the cases and controls respectively. A freshly-collected voided urine sample was divided into four parts, with each part used to perform: a) conventional cytology with Papanicolaou staining; b) the BTA-TRAK assay; c) the NMP-22 assay; and d) 5-ALA-induced fluorescent urine cytology. The resected bladder specimen was sent for histopathological examination. Results In low-grade bladder cancers, the sensitivity of 5-ALA fluorescent cytology was of 88.02%, which was significantly higher than conventional cytology (p < 0.0001), the NMP-22 assay (p < 0.0035), and the BTA-TRAK assay (p < 0.0007). The sensitivity of 5-ALA fluorescent cytology was significantly higher in high-grade lesions when compared to conventional cytology (p < 0.0005) and the BTA-TRAK assay (p < 0.039). Conclusions Fluorescent urine cytology induced by 5-aminolevulinic acid is a highly-sensitive test in the diagnosis of bladder cancer, and its sensitivity rates are significantly superior to those of conventional cytology, the NMP-22 assay, and the BTA-TRAK assay.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: 5-ALA-Induced Fluorescent Urine Cytology in Comparison with Conventional Cytology, BTA-TRAK, and NMP-22 Tests in the Diagnosis of Bladder Cancer ; volume:21 ; number:CP ; year:2025 ; Brazilian journal of oncology ; 21, Heft CP (2025)

Contributors, Places and Time

• Contributor: Nerli, Rajendra B. ; Rangrez, Shadab ; Ghagane, Shridhar C. ; Lalakia, Parth ; Chandra, Shoubhik ; Chandra, Shreya

Further information

• DOI: 10.1055/s-0044-1791752
• URN: urn:nbn:de:101:1-2503061017519.601530561155
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:30 AM CEST