DDX60 selectively reduces translation off viral type II internal ribosome entry sites
Abstract: Co‐opting host cell protein synthesis is a hallmark of many virus infections. In response, certain host defense proteins limit mRNA translation globally, albeit at the cost of the host cell's own protein synthesis. Here, we describe an interferon‐stimulated helicase, DDX60, that decreases translation from viral internal ribosome entry sites (IRESs). DDX60 acts selectively on type II IRESs of encephalomyocarditis virus (EMCV) and foot and mouth disease virus (FMDV), but not by other IRES types or by 5′ cap. Correspondingly, DDX60 reduces EMCV and FMDV (type II IRES) replication, but not that of poliovirus or bovine enterovirus 1 (BEV‐1; type I IRES). Furthermore, replacing the IRES of poliovirus with a type II IRES is sufficient for DDX60 to inhibit viral replication. Finally, DDX60 selectively modulates the amount of translating ribosomes on viral and in vitro transcribed type II IRES mRNAs, but not 5′ capped mRNA. Our study identifies a novel facet in the repertoire of interferon‐stimulated effector genes, the selective downregulation of translation from viral type II IRES elements.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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DDX60 selectively reduces translation off viral type II internal ribosome entry sites ; day:18 ; month:10 ; year:2022 ; extent:34
EMBO reports / European Molecular Biology Organization ; (18.10.2022) (gesamt 34)
- Urheber
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Sadic, Mohammad
Schneider, William M.
Katsara, Olga
Medina, Gisselle N.
Fisher, Ashley
Mogulothu, Aishwarya
Yu, Yingpu
Gu, Meigang
de los Santos, Teresa
Schneider, Robert J.
Dittmann, Meike
- DOI
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10.15252/embr.202255218
- URN
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urn:nbn:de:101:1-2022101915012640191508
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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2025-08-15T07:27:34+0200
Datenpartner
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Beteiligte
- Sadic, Mohammad
- Schneider, William M.
- Katsara, Olga
- Medina, Gisselle N.
- Fisher, Ashley
- Mogulothu, Aishwarya
- Yu, Yingpu
- Gu, Meigang
- de los Santos, Teresa
- Schneider, Robert J.
- Dittmann, Meike
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IRESpy: an XGBoost model for prediction of internal ribosome entry sites
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STIC2 selectively binds ribosome-nascent chain complexes in the cotranslational sorting of Arabidopsis thylakoid proteins
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