Transcription factor p63 bookmarks and regulates dynamic enhancers during epidermal differentiation
Abstract: The transcription factor p63 plays a pivotal role in keratinocyte proliferation and differentiation in the epidermis. However, how p63 regulates epidermal genes during differentiation is not yet clear. Using epigenome profiling of differentiating human primary epidermal keratinocytes, we characterized a catalog of dynamically regulated genes and p63‐bound regulatory elements that are relevant for epithelial development and related diseases. p63‐bound regulatory elements occur as single or clustered enhancers, and remarkably, only a subset is active as defined by the co‐presence of the active enhancer mark histone modification H3K27ac in epidermal keratinocytes. We show that the dynamics of gene expression correlates with the activity of p63‐bound enhancers rather than with p63 binding itself. The activity of p63‐bound enhancers is likely determined by other transcription factors that cooperate with p63. Our data show that inactive p63‐bound enhancers in epidermal keratinocytes may be active during the development of other epithelial‐related structures such as limbs and suggest that p63 bookmarks genomic loci during the commitment of the epithelial lineage and regulates genes through temporal‐ and spatial‐specific active enhancers.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Transcription factor p63 bookmarks and regulates dynamic enhancers during epidermal differentiation ; volume:16 ; number:7 ; year:2015 ; pages:863-878 ; extent:16
EMBO reports / European Molecular Biology Organization ; 16, Heft 7 (2015), 863-878 (gesamt 16)
- Creator
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Kouwenhoven, Evelyn N.
Oti, Martin
Niehues, Hanna
van Heeringen, Simon J.
Schalkwijk, Joost
Stunnenberg, Hendrik G.
van Bokhoven, Hans
Zhou, Huiqing
- DOI
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10.15252/embr.201439941
- URN
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urn:nbn:de:101:1-2022112305010958825723
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:36 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kouwenhoven, Evelyn N.
- Oti, Martin
- Niehues, Hanna
- van Heeringen, Simon J.
- Schalkwijk, Joost
- Stunnenberg, Hendrik G.
- van Bokhoven, Hans
- Zhou, Huiqing
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Probing transcription factor combinatorics in different promoter classes and in enhancers
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Bidirectional transcription initiation marks accessible chromatin and is not specific to enhancers
Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes
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Enhancers, enhancers – from their discovery to today’s universe of transcription enhancers
Master regulatory role of p63 in epidermal development and disease
Transcription Factor CTIP1/ BCL11A Regulates Epidermal Differentiation and Lipid Metabolism During Skin Development
Transcription through enhancers suppresses their activity in Drosophila
Super-enhancers complexes zoom in transcription in cancer
p63 transcriptionally regulates the expression of matrix metallopeptidase 13
Probing transcription factor combinatorics in different promoter classes and in enhancers
NLRP10 maintains epidermal homeostasis by promoting keratinocyte survival and P63-dependent differentiation and barrier function
Bidirectional transcription initiation marks accessible chromatin and is not specific to enhancers
Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes
Rho enhancers play unexpectedly minor roles in Rhodopsin transcription and rod cell integrity
Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome
Enhancers, enhancers – from their discovery to today’s universe of transcription enhancers
Master regulatory role of p63 in epidermal development and disease
Transcription Factor CTIP1/ BCL11A Regulates Epidermal Differentiation and Lipid Metabolism During Skin Development
Transcription through enhancers suppresses their activity in Drosophila
Super-enhancers complexes zoom in transcription in cancer
p63 transcriptionally regulates the expression of matrix metallopeptidase 13
Probing transcription factor combinatorics in different promoter classes and in enhancers
NLRP10 maintains epidermal homeostasis by promoting keratinocyte survival and P63-dependent differentiation and barrier function
Bidirectional transcription initiation marks accessible chromatin and is not specific to enhancers
Glucocorticoid receptor controls atopic dermatitis inflammation via functional interactions with P63 and autocrine signaling in epidermal keratinocytes
Rho enhancers play unexpectedly minor roles in Rhodopsin transcription and rod cell integrity