Long non-coding RNA-2271 promotes osteogenic differentiation in human bone marrow stem cells

Background: Human bone marrow mesenchymal stem cells (BMSCs) are of great significance for bone regeneration and bone formation. Long non-coding RNAs (lncRNAs) may be involved in modulating cell differentiation. This study aimed to investigate the role of lncR-2271 in promoting osteogenic differentiation in human BMSCs. Methods: Human BMSCs were infected using lncR-2271 overexpression (group A) with lentiviral system or transfected with lncR-2271 siRNA (group B). Cells transfected with scrambled plasmids were used as a negative control (group C). Osteogenesis markers were evaluated using alkaline phosphatase (ALP) activity, RUNX2 and osterix (OSX) at protein levels and calcification by Alizarin Red staining. Results: BMSCs from group A showed significantly higher ALP activity compared to BMSCs in group B and control group (group C) at both days 7 and 14 following osteogenic induction; ALP activity was significantly lower in the group B compared to the group C. RUNX2 and OSX protein expressions were significantly higher in group A and significantly lower in group B, compared to those in group C, respectively. At day 21, calcification in human BMSCs in group A was significantly higher compared to groups B and C as shown by Alizarin Red staining; calcification was significantly lower in group B compared to group C. Conclusion: Our data suggested lncR-2271 played a role in promoting osteogenic differentiation in human BMSCs. This study is the first to illustrate the important role of lncR-2271 in bone formation.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Long non-coding RNA-2271 promotes osteogenic differentiation in human bone marrow stem cells ; volume:13 ; number:1 ; year:2018 ; pages:404-412 ; extent:9 ; Open life sciences ; 13, Heft 1 (2018), 404-412 (gesamt 9)

Contributors, Places and Time

• Creator: Xi, Li-Cheng ; Li, Hong-Yu ; Yin, Dong

Further information

• DOI: 10.1515/biol-2018-0049
• URN: urn:nbn:de:101:1-2409201656294.237260318892
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 2025-08-15T07:35:13+0200