Characterization of the Nucleus Pulposus Progenitor Cells via Spatial Transcriptomics

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Abstract: Loss of refreshment in nucleus pulposus (NP) cellularity leads to intervertebral disc (IVD) degeneration. Nevertheless, the cellular sequence of NP cell differentiation remains unclear, although an increasing body of literature has identified markers of NP progenitor cells (NPPCs). Notably, due to their fragility, the physical enrichment of NP‐derived cells has limited conventional transcriptomic approaches in multiple studies. To overcome this limitation, a spatially resolved transcriptional atlas of the mouse IVD is generated via the 10x Genomics Visium platform dividing NP spots into two clusters. Based on this, most reported NPPC‐markers, including Cathepsin K (Ctsk), are rare and predominantly located within the NP‐outer subset. Cell lineage tracing further evidence that a small number of Ctsk‐expressing cells generate the entire adult NP tissue. In contrast, Tie2, which has long suggested labeling NPPCs, is actually neither expressed in NP subsets nor labels NPPCs and their descendants in mouse models; consistent with this, an in situ sequencing (ISS) analysis validated the absence of Tie2 in NP tissue. Similarly, no Tie2‐cre‐mediated labeling of NPPCs is observed in an IVD degenerative mouse model. Altogether, in this study, the first spatial transcriptomic map of the IVD is established, thereby providing a public resource for bone biology.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Characterization of the Nucleus Pulposus Progenitor Cells via Spatial Transcriptomics ; day:04 ; month:02 ; year:2024 ; extent:17
Advanced science ; (04.02.2024) (gesamt 17)

Creator: Chen, Yu
Zhang, Long
Shi, Xueqing
Han, Jie
Chen, Jingyu
Zhang, Xinya
Xie, Danlin
Li, Zan
Niu, Xing
Chen, Lijie
Yang, Chaoyong
Sun, Xiujie
Zhou, Taifeng
Su, Peiqiang
Li, Na
Greenblatt, Matthew B.
Ke, Rongqin
Huang, Jianming
Chen, Zhe‐Sheng
Xu, Ren

DOI: 10.1002/advs.202303752

URN: urn:nbn:de:101:1-2024020514074727915848

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:33 AM CEST

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Chen, Yu
Zhang, Long
Shi, Xueqing
Han, Jie
Chen, Jingyu
Zhang, Xinya
Xie, Danlin
Li, Zan
Niu, Xing
Chen, Lijie
Yang, Chaoyong
Sun, Xiujie
Zhou, Taifeng
Su, Peiqiang
Li, Na
Greenblatt, Matthew B.
Ke, Rongqin
Huang, Jianming
Chen, Zhe‐Sheng
Xu, Ren

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Characterization of the Nucleus Pulposus Progenitor Cells via Spatial Transcriptomics

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