Microfluidic Organoid Cultures Derived from Pancreatic Cancer Biopsies for Personalized Testing of Chemotherapy and Immunotherapy

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Abstract: Patient‐derived cancer organoids (PDOs) hold considerable promise for personalizing therapy selection and improving patient outcomes. However, it is challenging to generate PDOs in sufficient numbers to test therapies in standard culture platforms. This challenge is particularly acute for pancreatic ductal adenocarcinoma (PDAC) where most patients are diagnosed at an advanced stage with non‐resectable tumors and where patient tissue is in the form of needle biopsies. Here the development and characterization of microfluidic devices for testing therapies using a limited amount of tissue or PDOs available from PDAC biopsies is described. It is demonstrated that microfluidic PDOs are phenotypically and genotypically similar to the gold‐standard Matrigel organoids with the advantages of 1) spheroid uniformity, 2) minimal cell number requirement, and 3) not relying on Matrigel. The utility of microfluidic PDOs is proven by testing PDO responses to several chemotherapies, including an inhibitor of glycogen synthase kinase (GSKI). In addition, microfluidic organoid cultures are used to test effectiveness of immunotherapy comprised of NK cells in combination with a novel biologic. In summary, our microfluidic device offers considerable benefits for personalizing oncology based on cancer biopsies and may, in the future, be developed into a companion diagnostic for chemotherapy or immunotherapy treatments.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
Microfluidic Organoid Cultures Derived from Pancreatic Cancer Biopsies for Personalized Testing of Chemotherapy and Immunotherapy ; day:29 ; month:11 ; year:2023 ; extent:17
Advanced science ; (29.11.2023) (gesamt 17)

Creator
Choi, Daheui
Gonzalez‐Suarez, Alan M.
Dumbrava, Mihai G.
Medlyn, Michael
de Hoyos‐Vega, Jose M.
Cichocki, Frank
Miller, Jeffrey S.
Ding, Li
Zhu, Mojun
Stybayeva, Gulnaz
Gaspar‐Maia, Alexandre
Billadeau, Daniel D.
Ma, Wen Wee
Revzin, Alexander

DOI
10.1002/advs.202303088
URN
urn:nbn:de:101:1-2023112914303037035293
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:32 AM CEST

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Associated

  • Choi, Daheui
  • Gonzalez‐Suarez, Alan M.
  • Dumbrava, Mihai G.
  • Medlyn, Michael
  • de Hoyos‐Vega, Jose M.
  • Cichocki, Frank
  • Miller, Jeffrey S.
  • Ding, Li
  • Zhu, Mojun
  • Stybayeva, Gulnaz
  • Gaspar‐Maia, Alexandre
  • Billadeau, Daniel D.
  • Ma, Wen Wee
  • Revzin, Alexander

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