Cytolinker Gas2L1 regulates axon morphology through microtubule‐modulated actin stabilization
Abstract: Crosstalk between the actin and microtubule cytoskeletons underlies cellular morphogenesis. Interactions between actin filaments and microtubules are particularly important for establishing the complex polarized morphology of neurons. Here, we characterized the neuronal function of growth arrest‐specific 2‐like 1 (Gas2L1), a protein that can directly bind to actin, microtubules and microtubule plus‐end‐tracking end binding proteins. We found that Gas2L1 promotes axon branching, but restricts axon elongation in cultured rat hippocampal neurons. Using pull‐down experiments and in vitro reconstitution assays, in which purified Gas2L1 was combined with actin and dynamic microtubules, we demonstrated that Gas2L1 is autoinhibited. This autoinhibition is relieved by simultaneous binding to actin filaments and microtubules. In neurons, Gas2L1 primarily localizes to the actin cytoskeleton and functions as an actin stabilizer. The microtubule‐binding tail region of Gas2L1 directs its actin‐stabilizing activity towards the axon. We propose that Gas2L1 acts as an actin regulator, the function of which is spatially modulated by microtubules.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Cytolinker Gas2L1 regulates axon morphology through microtubule‐modulated actin stabilization ; volume:20 ; number:11 ; year:2019 ; extent:20
EMBO reports / European Molecular Biology Organization ; 20, Heft 11 (2019) (gesamt 20)
- Creator
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van de Willige, Dieudonnée
Hummel, Jessica JA
Alkemade, Celine
Kahn, Olga I.
Au, Franco KC
Qi, Robert Z.
Dogterom, Marileen
Koenderink, Gijsje H.
Hoogenraad, Casper C.
Akhmanova, Anna
- DOI
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10.15252/embr.201947732
- URN
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urn:nbn:de:101:1-2022081208404734787977
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:33 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- van de Willige, Dieudonnée
- Hummel, Jessica JA
- Alkemade, Celine
- Kahn, Olga I.
- Au, Franco KC
- Qi, Robert Z.
- Dogterom, Marileen
- Koenderink, Gijsje H.
- Hoogenraad, Casper C.
- Akhmanova, Anna
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