Development and validation of the stability-indicating LC-UV method for the determination of cefoselis sulphate

Abstract: The stability-indicating LC assay method was developed and validated for quantitative determination of cefoselis sulphate in the presence of degradation products formed during the forced degradation studies. An isocratic, RP-HPLC method was developed with C-18 (250 × 4.6 mm, 5 µm) column and 12 mM ammonium acetate-acetonitrile (95:5 V/V) as a mobile phase. The flow rate of the mobile phase was 1.0 mL min−1. Detection wavelength was 260 nm and temperature was 30°C. Cefoselis similarly to other cephalosporins was subjected to stress conditions of degradation in aqueous solutions including hydrolysis, oxidation, photolysis and thermal degradation. The developed method was validated with regard to linearity, accuracy, precision, selectivity and robustness. The method was applied successfully for identification and determination of cefoselis sulphate in pharmaceuticals and during kinetic studies.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Development and validation of the stability-indicating LC-UV method for the determination of cefoselis sulphate ; volume:10 ; number:1 ; year:2012 ; pages:121-126 ; extent:6 ; Open chemistry ; 10, Heft 1 (2012), 121-126 (gesamt 6)

Contributors, Places and Time

• Creator: Zalewski, Przemysław ; Cielecka-Piontek, Judyta ; Jelińska, Anna

Further information

• DOI: 10.2478/s11532-011-0112-9
• URN: urn:nbn:de:101:1-2410181711480.593921686554
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:36 AM CEST