Universal LIMS based platform for the automated processing of cell-based assays
Abstract: Nowadays, cell-based assays are an elementary tool for diagnostics, animal-free substance testing and basic research. Depending on the application, the spectrum ranges from simple static cell cultures in microtiter plates to dynamic co-cultures in complex micro physiological systems (organ-on-a-chip). Depending on the complexity of the assay, numerous working steps have to be performed and the data from different analysis systems have to be processed, combined and documented. A universal platform has been developed for the automated handling of cell-based assays, which combines a laboratory information management system (LIMS) with a laboratory execution system (LES), a universal laboratory automation platform and established laboratory equipment. The LIMS handles the administration of all laboratory-relevant information, the planning, control and monitoring of laboratory processes, as well as the direct and qualified processing of raw data. Using a kidney-on-achip system as an example, the realization of complex cellbased assays for the animal-free characterization of the toxicity of different antibiotics will be demonstrated. In the kidney-on-a-chip system the artificial proximal tubular barrier was formed by seeding human immortalized proximal tubule cells (RPTEC) and human blood outgrowth endothelial cells (BOEC) on ThinCert™ membranes. Transepithelial electrical resistance (TEER) was measured daily to evaluate the barrier function of the cellular layers. Fluid handling and TEER measurements were performed using a laboratory automation platform that communicates directly with the LIMS. The LES supports laboratory assistants in executing the manual handling steps of the experiments.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Universal LIMS based platform for the automated processing of cell-based assays ; volume:5 ; number:1 ; year:2019 ; pages:437-439 ; extent:4
Current directions in biomedical engineering ; 5, Heft 1 (2019), 437-439 (gesamt 4)
- Creator
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Schmieder, Florian
Polk, Christoph
Gottlöber, Felix
Schöps, Patrick
Sonntag, Frank
Deuse, Ronny
Jede, Aline
Petzold, Thomas
- DOI
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10.1515/cdbme-2019-0110
- URN
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urn:nbn:de:101:1-2022101214570608100848
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:29 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Schmieder, Florian
- Polk, Christoph
- Gottlöber, Felix
- Schöps, Patrick
- Sonntag, Frank
- Deuse, Ronny
- Jede, Aline
- Petzold, Thomas
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