Supramolecular Control over Split‐Luciferase Complementation
Abstract: Supramolecular split‐enzyme complementation restores enzymatic activity and allows for on–off switching. Split‐luciferase fragment pairs were provided with an N‐terminal FGG sequence and screened for complementation through host‐guest binding to cucurbit[8]uril (Q8). Split‐luciferase heterocomplex formation was induced in a Q8 concentration dependent manner, resulting in a 20‐fold upregulation of luciferase activity. Supramolecular split‐luciferase complementation was fully reversible, as revealed by using two types of Q8 inhibitors. Competition studies with the weak‐binding FGG peptide revealed a 300‐fold enhanced stability for the formation of the ternary heterocomplex compared to binding of two of the same fragments to Q8. Stochiometric binding by the potent inhibitor memantine could be used for repeated cycling of luciferase activation and deactivation in conjunction with Q8, providing a versatile module for in vitro supramolecular signaling networks.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Supramolecular Control over Split‐Luciferase Complementation ; volume:128 ; number:31 ; year:2016 ; pages:9045-9049 ; extent:5
Angewandte Chemie ; 128, Heft 31 (2016), 9045-9049 (gesamt 5)
- Creator
-
Bosmans, Ralph P. G.
Briels, Jeroen M.
Milroy, Lech‐Gustav
de Greef, Tom F. A.
Merkx, Maarten
Brunsveld, Luc
- DOI
-
10.1002/ange.201602807
- URN
-
urn:nbn:de:101:1-2022110106253461755186
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:26 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Bosmans, Ralph P. G.
- Briels, Jeroen M.
- Milroy, Lech‐Gustav
- de Greef, Tom F. A.
- Merkx, Maarten
- Brunsveld, Luc
Other Objects (12)
Supramolecular Control over Split‐Luciferase Complementation
Split-Luciferase Complementation and Molecular Dynamics Studies for the Mini-G Protein-Based Functional Characterization of GPCRs
Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
Luciferase complementation for the determination of arrestin recruitment: Investigations at histamine and NPY receptors
Complementation
ComplémentationS
ComplémentationS
Subgraph Complementation
A self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion
A widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase
Tetramolecular fluorescence complementation
Supramolecular Control over Split‐Luciferase Complementation
Split-Luciferase Complementation and Molecular Dynamics Studies for the Mini-G Protein-Based Functional Characterization of GPCRs
Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
Luciferase complementation for the determination of arrestin recruitment: Investigations at histamine and NPY receptors
Complementation
ComplémentationS
ComplémentationS
Subgraph Complementation
A self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion
A widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase
Tetramolecular fluorescence complementation
Supramolecular Control over Split‐Luciferase Complementation
Split-Luciferase Complementation and Molecular Dynamics Studies for the Mini-G Protein-Based Functional Characterization of GPCRs
Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments
Luciferase complementation for the determination of arrestin recruitment: Investigations at histamine and NPY receptors
Complementation
ComplémentationS
ComplémentationS
Subgraph Complementation
A self-assembling split Nano luciferase-based assay for investigating Pseudomonas syringae effector secretion
A widely-applicable high-throughput cellular thermal shift assay (CETSA) using split Nano Luciferase