Bioorthogonal Ligation‐Activated Fluorogenic FRET Dyads
Abstract: An energy transfer‐based signal amplification relay concept enabling transmission of bioorthogonally activatable fluorogenicity of blue‐excitable coumarins to yellow/red emitting cyanine frames is presented. Such relay mechanism resulted in improved cyanine fluorogenicities together with increased photostabilities and large apparent Stokes‐shifts allowing lower background fluorescence even in no‐wash bioorthogonal fluorogenic labeling schemes of intracellular structures in live cells. These energy transfer dyads sharing the same donor moiety together with their parent donor molecule allowed three‐color imaging of intracellular targets using one single excitation source with separate emission windows. Sub‐diffraction imaging of intracellular structures using the bioorthogonally activatable FRET dyads by STED microscopy is also presented.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Bioorthogonal Ligation‐Activated Fluorogenic FRET Dyads ; day:27 ; month:12 ; year:2021 ; extent:8
Angewandte Chemie / International edition. International edition ; (27.12.2021) (gesamt 8)
- Creator
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Albitz, Evelin
Kern, Dóra
Kormos, Attila
Bojtár, Márton
Török, György
Biró, Adrienn
Szatmári, Ágnes
Németh, Krisztina
Kele, Péter
- DOI
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10.1002/anie.202111855
- URN
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urn:nbn:de:101:1-2021122814144966644031
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
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Associated
- Albitz, Evelin
- Kern, Dóra
- Kormos, Attila
- Bojtár, Márton
- Török, György
- Biró, Adrienn
- Szatmári, Ágnes
- Németh, Krisztina
- Kele, Péter
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Bioorthogonal reactions of heterodienes: synthesis, reactivity and fluorogenic bioimaging applications
Chemoselective and bioorthogonal ligation reactions, Volume 1
Chemoselective and bioorthogonal ligation reactions, Volume 2
Recent Advances in Bioorthogonal Ligation and Bioconjugation
Photoactivatable Fluorogenic Labeling via Turn‐On “Click‐Like” Nitroso‐Diene Bioorthogonal Reaction
A Dansyl Amide N ‐Oxide Fluorogenic Probe Based on a Bioorthogonal Decaging Reaction
Bioorthogonal metal-free click-ligation of cRGD-pentapeptide to alginate
Staudinger Ligation and Reactions – From Bioorthogonal Labeling to Next‐Generation Biopharmaceuticals
Staudinger Ligation and Reactions – From Bioorthogonal Labeling to Next‐Generation Biopharmaceuticals
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Bioorthogonal Ligation‐Activated Fluorogenic FRET Dyads
Bioorthogonal reactions of heterodienes: synthesis, reactivity and fluorogenic bioimaging applications
Chemoselective and bioorthogonal ligation reactions, Volume 1
Chemoselective and bioorthogonal ligation reactions, Volume 2
Recent Advances in Bioorthogonal Ligation and Bioconjugation
Photoactivatable Fluorogenic Labeling via Turn‐On “Click‐Like” Nitroso‐Diene Bioorthogonal Reaction
A Dansyl Amide N ‐Oxide Fluorogenic Probe Based on a Bioorthogonal Decaging Reaction
Bioorthogonal metal-free click-ligation of cRGD-pentapeptide to alginate
Staudinger Ligation and Reactions – From Bioorthogonal Labeling to Next‐Generation Biopharmaceuticals
Staudinger Ligation and Reactions – From Bioorthogonal Labeling to Next‐Generation Biopharmaceuticals
A Bioorthogonal Dual Fluorogenic Probe for the Live‐Cell Monitoring of Nutrient Uptake by Mammalian Cells