A Novel Pathway of Functional microRNA Uptake and Mitochondria Delivery
Abstract: Extracellular microRNAs (miRNAs) play a critical role in horizontal gene regulation. Uptake of extracellular miRNAs by recipient cells and their intracellular transport, however, remains elusive. Here RNA phase separation is shown as a novel pathway of miRNA uptake. In the presence of serum, synthetic miRNAs rapidly self‐assembly into ≈110 nm discrete nanoparticles, which enable miRNAs’ entry into different cells. Depleting serum cationic proteins prevents the formation of such nanoparticles and thus blocks miRNA uptake. Different from lipofectamine‐mediated miRNA transfection in which majority of miRNAs are accumulated in lysosomes of transfected cells, nanoparticles‐mediated miRNA uptake predominantly delivers miRNAs into mitochondria in a polyribonucleotide nucleotidyltransferase 1 (PNPT1)‐dependent manner. Functional assays further show that the internalized miR‐21 via miRNA phase separation enhances mitochondrial translation of cytochrome b (CYB), leading to increase in adenosine triphosphate (ATP) and reactive oxygen species (ROS) reduction in HEK293T cells. The findings thus reveal a previously unrecognized mechanism for uptake and delivery functional extracellular miRNAs into mitochondria.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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A Novel Pathway of Functional microRNA Uptake and Mitochondria Delivery ; day:25 ; month:06 ; year:2023 ; extent:12
Advanced science ; (25.06.2023) (gesamt 12)
- Creator
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Liu, Jiachen
Li, Weili
Li, Jianfeng
Song, Eli
Liang, Hongwei
Rong, Weiwei
Jiang, Xinli
Xu, Nuo
Wang, Wei
Qu, Shuang
Gu, Shouyong
Zhang, Yujing
Yu Zhang, Chen‐
Zen, Ke
- DOI
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10.1002/advs.202300452
- URN
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urn:nbn:de:101:1-2023062615071585755937
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:49 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Liu, Jiachen
- Li, Weili
- Li, Jianfeng
- Song, Eli
- Liang, Hongwei
- Rong, Weiwei
- Jiang, Xinli
- Xu, Nuo
- Wang, Wei
- Qu, Shuang
- Gu, Shouyong
- Zhang, Yujing
- Yu Zhang, Chen‐
- Zen, Ke
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