(Pre) analytical considerations concerning the analysis of synovial calprotectin

Objectives: Several studies have demonstrated that synovial calprotectin is a highly accurate biomarker in diagnosing periprosthetic joint infections (PJI). Assuring reliability is of great importance and coincides with adequate preanalytical handling. This study focuses on potentially interfering factors. Methods: To assess the stability of synovial calprotectin, the effect of time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination was investigated. In the blood and lipid contamination experiments, hemolyzed and non-hemolyzed blood, homogenized adipose tissue, intralipid and chylomicrons were added. The effect of viscosity was investigated using freeze-thaw cycles, enzymatic pretreatment and sonification. Results: No effect on synovial calprotectin levels was observed in synovial samples kept at room temperature compared to samples kept at 4 °C for up to seven days of storage. Freeze-thaw cycles did not result in significantly different calprotectin levels, although samples without EDTA resulted in higher recoveries after 1 and 2 freeze-thaw cycles. Blood and lipid contamination did not interfere with accurate synovial calprotectin analysis. Sample pretreatment to reduce sample viscosity by pretreating samples with DNAse and/or hyaluronidase did not influence calprotectin analysis. Sonification, however, resulted in increased calprotectin values. Conclusions: Synovial calprotectin is a stable biomarker and its analysis is not easily influenced by potential interfering factors.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: (Pre) analytical considerations concerning the analysis of synovial calprotectin ; volume:62 ; number:1 ; year:2024 ; pages:199-206 ; extent:08 ; Clinical chemistry and laboratory medicine ; 62, Heft 1 (2024), 199-206 (gesamt 08)

Contributors, Places and Time

• Creator: Alkadhem, Mohammed F. ; Wagenmakers-Huizenga, Lucie M.F. ; Wouthuyzen-Bakker, Marjan ; Muller Kobold, Anneke C.

Further information

• DOI: 10.1515/cclm-2023-0484
• URN: urn:nbn:de:101:1-2023112713452715039010
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:39 AM CEST