Buformin suppresses osteosarcoma via targeting AMPK signaling pathway
Background: Buformin has been reported to be a powerful anticancer drug by activating the AMPK signal. Herein, we aimed to investigate the effects of buformin on osteosarcoma. Material and methods: Cellular proliferative abilities were determined by cell counting kit-8 and colony formation assays. Cellular invasion was investigated using a transwell system. Cell cycle was examined by flow cytometry. Western blot was performed to measure the expression of key proteins. Synergistic effects of buformin and cisplatin were validated in seven fresh osteosarcoma tissues. Results: Buformin suppressed the growth of U-2 OS cells in a dose-dependent manner (IC50 = 69.1 µM). Moreover, buformin induced cell cycle arrest (P < 0.001) and impaired cellular invasion (P = 0.038). Phosphorylation of AMPK was upregulated by buformin, while phosphorylation of S6, cyclin D1, and MMP9 were significantly downregulated. In addition, buformin notably induced accumulation of reactive oxygen species and lactate and eventually decreased ATP production. In both U-2 OS cells and the primary cultured osteosarcoma tissues, buformin increased tumor sensitivity to cisplatin. Conclusions: Buformin could suppress tumor growth and invasion of osteosarcoma through directly targeting the AMPK signaling pathway. Moreover, buformin inhibited the abnormal metabolism and notably increased the cytotoxicity of cisplatin, and therefore represents a new potential treatment option for osteosarcoma.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Buformin suppresses osteosarcoma via targeting AMPK signaling pathway ; volume:15 ; number:1 ; year:2020 ; pages:409-417 ; extent:9
Open life sciences ; 15, Heft 1 (2020), 409-417 (gesamt 9)
- Urheber
-
Ding, Yan
Lv, Shiqiao
Li, Guangrun
Cui, Jinpeng
Chen, Yunzhen
- DOI
-
10.1515/biol-2020-0041
- URN
-
urn:nbn:de:101:1-2409201746428.389892337052
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:20 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Ding, Yan
- Lv, Shiqiao
- Li, Guangrun
- Cui, Jinpeng
- Chen, Yunzhen
Ähnliche Objekte (12)
Mir-615-3p promotes osteosarcoma progression via the SESN2/AMPK/mTOR pathway
Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway
Licochalcone Suppresses LXRα-Induced Hepatic Lipogenic Gene Expression through AMPK/Sirt1 Pathway Activation
miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4
MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway
MiR-29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6
Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway
Targeting iron metabolism in osteosarcoma
Cordycepin augments the chemosensitivity of osteosarcoma to cisplatin by activating AMPK and suppressing the AKT signaling pathway
Chitosan oligosaccharide suppresses osteosarcoma malignancy by inhibiting CEMIP via the PI3K/AKT/mTOR pathway
miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2
Over-expression of hsa_circ_0088214 suppresses tumor progression by inhibiting Akt signaling pathway in osteosarcoma
Mir-615-3p promotes osteosarcoma progression via the SESN2/AMPK/mTOR pathway
Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway
Licochalcone Suppresses LXRα-Induced Hepatic Lipogenic Gene Expression through AMPK/Sirt1 Pathway Activation
miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4
MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway
MiR-29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6
Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway
Targeting iron metabolism in osteosarcoma
Cordycepin augments the chemosensitivity of osteosarcoma to cisplatin by activating AMPK and suppressing the AKT signaling pathway
Chitosan oligosaccharide suppresses osteosarcoma malignancy by inhibiting CEMIP via the PI3K/AKT/mTOR pathway
miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2
Over-expression of hsa_circ_0088214 suppresses tumor progression by inhibiting Akt signaling pathway in osteosarcoma
Mir-615-3p promotes osteosarcoma progression via the SESN2/AMPK/mTOR pathway
Adiponectin suppresses tumor growth of nasopharyngeal carcinoma through activating AMPK signaling pathway
Licochalcone Suppresses LXRα-Induced Hepatic Lipogenic Gene Expression through AMPK/Sirt1 Pathway Activation
miR-3195 suppresses the malignant progression of osteosarcoma cells via targeting SOX4
MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway
MiR-29b suppresses the proliferation and migration of osteosarcoma cells by targeting CDK6
Avermectin B1 mediates antitumor activity and induces autophagy in osteosarcoma through the AMPK/ULK1 signaling pathway
Targeting iron metabolism in osteosarcoma
Cordycepin augments the chemosensitivity of osteosarcoma to cisplatin by activating AMPK and suppressing the AKT signaling pathway
Chitosan oligosaccharide suppresses osteosarcoma malignancy by inhibiting CEMIP via the PI3K/AKT/mTOR pathway
miR-194 Suppresses Proliferation and Migration and Promotes Apoptosis of Osteosarcoma Cells by Targeting CDH2