Nucleic Acid‐based Enzyme Cascades—Current Trends and Future Perspectives

Abstract: The natural micro‐ and nanoscale organization of biomacromolecules is a remarkable principle within living cells, allowing for the control of cellular functions by compartmentalization, dimensional diffusion and substrate channeling. In order to explore these biological mechanisms and harness their potential for applications such as sensing and catalysis, molecular scaffolding has emerged as a promising approach. In the case of synthetic enzyme cascades, developments in DNA nanotechnology have produced particularly powerful scaffolds whose addressability can be programmed with nanometer precision. In this minireview, we summarize recent developments in the field of biomimetic multicatalytic cascade reactions organized on DNA nanostructures. We emphasize the impact of the underlying design principles like DNA origami, efficient strategies for enzyme immobilization, as well as the importance of experimental design parameters and theoretical modeling. We show how DNA nanostructures have enabled a better understanding of diffusion and compartmentalization effects at the nanometer length scale, and discuss the challenges and future potential for commercial applications.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Nucleic Acid‐based Enzyme Cascades—Current Trends and Future Perspectives ; day:20 ; month:11 ; year:2023 ; extent:10 ; Angewandte Chemie / International edition. International edition ; (20.11.2023) (gesamt 10)

Beteiligte, Orts- und Zeitangaben

• Urheber: Kröll, Sandra ; Niemeyer, Christof M.

Weitere Informationen

• DOI: 10.1002/anie.202314452
• URN: urn:nbn:de:101:1-2023112114551769098260
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:21 MESZ