A Photoreceptor‐Based Hydrogel with Red Light‐Responsive Reversible Sol‐Gel Transition as Transient Cellular Matrix

Abstract: Hydrogels with adjustable mechanical properties have been engineered as matrices for mammalian cells and allow the dynamic, mechano‐responsive manipulation of cell fate and function. Recent research yields hydrogels, where biological photoreceptors translated optical signals into a reversible and adjustable change in hydrogel mechanics. While their initial application provides important insights into mechanobiology, broader implementation is limited by a small dynamic range of addressable stiffness. Herein, this limitation is overcome by developing a photoreceptor‐based hydrogel with reversibly adjustable stiffness from ≈800 Pa to the sol state. The hydrogel is based on star‐shaped polyethylene glycol, functionalized with the red/far‐red light photoreceptor phytochrome B (PhyB), or phytochrome‐interacting factor 6 (PIF6). Upon illumination with red light, PhyB heterodimerizes with PIF6, thus crosslinking the polymers and resulting in gelation. However, upon illumination with far‐red light, the proteins dissociate and trigger a complete gel‐to‐sol transition. The hydrogel's light‐responsive mechanical properties are comprehensively characterized and it is applied as a reversible extracellular matrix for the spatiotemporally controlled deposition of mammalian cells within a microfluidic chip. It is anticipated that this technology will open new avenues for the site‐ and time‐specific positioning of cells and will contribute to overcome spatial restrictions.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: A Photoreceptor‐Based Hydrogel with Red Light‐Responsive Reversible Sol‐Gel Transition as Transient Cellular Matrix ; day:18 ; month:06 ; year:2023 ; extent:10 ; Advanced Materials Technologies ; (18.06.2023) (gesamt 10)

Contributors, Places and Time

• Creator: Hörner, Maximilian ; Becker, Jan ; Bohnert, Rebecca ; Baños, Miguel ; Jerez‐Longres, Carolina ; Mühlhäuser, Vanessa ; Härrer, Daniel ; Wong, Tin Wang ; Meier, Matthias ; Weber, Wilfried

Further information

• DOI: 10.1002/admt.202300195
• URN: urn:nbn:de:101:1-2023061915060112132580
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:57 AM CEST