Assay for Characterizing Adsorption‐Properties of Surfaces (APS)

Abstract: Analytes, from sample preparation, until entering an analytical instrument, are prone to adsorb to surfaces, driven by the chemical properties of the surface and the liquids they are dissolved in. This problem can be addressed with internal standards when a single or few known analytes are quantified that are usually not available in omics. However, minimal to no loss of analytes is the aim. Here, we present a novel assay for qualifying and quantifying interactions responsible for adsorption of molecules to surfaces (APS) by using LC–MS/MS‐based differential quantitative analysis. To reflect a broad range of chemical interactions with surfaces, a reference mixture of thousands of tryptic peptides, with known compositions was selected, representing a variety of different chemical characteristics. The assay was tested by investigating the adsorption properties of several different vials with different surface chemistries. A significant number of hydrophobic peptides adsorbed to conventional polypropylene vials. In contrast, only few peptides adsorbed to polypropylene vials, assigned as low‐protein‐binding. The highest number of peptides adsorbed to glass vials driven by electrostatic interactions. In summary, the new assay is suitable to characterize adsorption properties of different surfaces and to approximate the loss of analytes during sample preparation.

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch

Bibliographic citation
Assay for Characterizing Adsorption‐Properties of Surfaces (APS) ; day:16 ; month:10 ; year:2024 ; extent:10
Chemistry - a European journal ; (16.10.2024) (gesamt 10)

Creator
Siebels, Bente
Moritz, Manuela
Hübler, Diana
Gocke, Antonia
Riedner, Maria
Voß, Hannah
Schlüter, Hartmut

DOI
10.1002/chem.202403000
URN
urn:nbn:de:101:1-2410171420524.040371215283
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:27 AM CEST

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Associated

  • Siebels, Bente
  • Moritz, Manuela
  • Hübler, Diana
  • Gocke, Antonia
  • Riedner, Maria
  • Voß, Hannah
  • Schlüter, Hartmut

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