Impact of C‐terminal amino acid composition on protein expression in bacteria

to related objects

Abstract: The C‐terminal sequence of a protein is involved in processes such as efficiency of translation termination and protein degradation. However, the general relationship between features of this C‐terminal sequence and levels of protein expression remains unknown. Here, we identified C‐terminal amino acid biases that are ubiquitous across the bacterial taxonomy (1,582 genomes). We showed that the frequency is higher for positively charged amino acids (lysine, arginine), while hydrophobic amino acids and threonine are lower. We then studied the impact of C‐terminal composition on protein levels in a library of Mycoplasma pneumoniae mutants, covering all possible combinations of the two last codons. We found that charged and polar residues, in particular lysine, led to higher expression, while hydrophobic and aromatic residues led to lower expression, with a difference in protein levels up to fourfold. We further showed that modulation of protein degradation rate could be one of the main mechanisms driving these differences. Our results demonstrate that the identity of the last amino acids has a strong influence on protein expression levels.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Impact of C‐terminal amino acid composition on protein expression in bacteria ; volume:16 ; number:5 ; year:2020 ; extent:19
Molecular systems biology ; 16, Heft 5 (2020) (gesamt 19)

Creator: Weber, Marc
Burgos, Raul
Yus, Eva
Yang, Jae‐Seong
Lluch‐Senar, Maria
Serrano, Luis

DOI: 10.15252/msb.20199208

URN: urn:nbn:de:101:1-2022062408085739990448

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:22 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Weber, Marc
Burgos, Raul
Yus, Eva
Yang, Jae‐Seong
Lluch‐Senar, Maria
Serrano, Luis

Other Objects (12)

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.

Impact of C‐terminal amino acid composition on protein expression in bacteria

Object Details

References and Relationships

Contributors, Places and Time

Further information

Data provider

Associated

Other Objects (12)

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Binaphthyl-anchored antibacterial tripeptide derivatives with hydrophobic C-terminal amino acid variations

C-terminal amino acids are essential for human heat shock protein 70 dimerization

Beyond the C-terminal glycine of ATG8 proteins – The story of some neglected amino acids

Ectopic expression of C-terminal tubulin variants alters wood composition and structure in Populus

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Suppressive cancer nonstop extension mutations increase C-terminal hydrophobicity and disrupt evolutionarily conserved amino acid patterns

Cotranslational folding of HemK C-terminal domain

Methionine-associated peptide α-amidation is directed both to the N- and the C-terminal amino acids

Identification of two Amino Acids in the C-terminal Domain of Mouse CRY2 Essential for PER2 Interaction

Asxl1 C-terminal mutation perturbs neutrophil differentiation in zebrafish

Structural Dynamics of the MscL C-terminal Domain

Epigenetic modulation via the C-terminal tail of H2A.Z

Related objects

Reset password