Rapid Thermal Drying Synthesis of Nonthiolated Spherical Nucleic Acids with Stability Rivaling Thiolated DNA
Abstract: Attaching DNA oligonucleotides to gold nanoparticles (AuNPs) to prepare spherical nucleic acids (SNAs) has offered tremendous insights into surface chemistry with resulting bioconjugates serving as critical reagents in biosensors and nanotechnology. While thiolated DNA is generally required to achieve stable conjugates, we herein communicate that using a thermal drying method, a high DNA density and excellent SNA stability was achieved using nonthiolated DNA, rivaling the performance of thiolated DNA such as surviving 1 M NaCl, 2 month stability in 0.3 M NaCl and working in 50 % serum. A poly‐adenine block with as few as two consecutive terminal adenine bases is sufficient for anchoring on AuNPs. By side‐by‐side comparison with the salt‐aging method, the conjugation mechanism was attributed to competitive adenine adsorption at high temperature along with an extremely high DNA concentration upon drying. Bioanalytical applications of nonthiolated SNAs were validated in both solution and paper‐based sensor platforms, facilitating cost‐effective applications for SNAs.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Rapid Thermal Drying Synthesis of Nonthiolated Spherical Nucleic Acids with Stability Rivaling Thiolated DNA ; day:22 ; month:10 ; year:2024 ; extent:6
Angewandte Chemie / International edition. International edition ; (22.10.2024) (gesamt 6)
- Creator
- DOI
-
10.1002/anie.202410353
- URN
-
urn:nbn:de:101:1-2410231447473.928720123755
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
16.04.2032, 11:24 PM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Wang, Xin
- Yang, Zhansen
- Li, Zihe
- Huang, Kunlun
- Cheng, Nan
- Liu, Juewen
Other Objects (12)
Rapid Thermal Drying Synthesis of Nonthiolated Spherical Nucleic Acids with Stability Rivaling Thiolated DNA
Kompakte Mehrtorantennen für die adaptive Keulenformung
Chinese Food Network in British Columbia, 1880-1940 : A Socio-economic History of Migrant Entrepreneurship
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Development of an improved two-sphere integration technique for quantifying black carbon concentrations in the atmosphere and seasonal snow
Hydrothermal synthesis and crystal structure of catena -poly (diaqua-bis (μ 2 -4-((pyridin-4-ylmethyl) amino) benzoato-κ 2 N:O) cobalt (II)) – 4,4′-bipyridine – water (1/2/2)
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Muon (g − 2) and flavor puzzles in the U(1) X -gauged leptoquark model
A class of stochastic Gronwall’s inequality and its application
Efficient Feature Matching and Pose-graph Initialization for SfM
Rapid Thermal Drying Synthesis of Nonthiolated Spherical Nucleic Acids with Stability Rivaling Thiolated DNA
Kompakte Mehrtorantennen für die adaptive Keulenformung
Chinese Food Network in British Columbia, 1880-1940 : A Socio-economic History of Migrant Entrepreneurship
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Development of an improved two-sphere integration technique for quantifying black carbon concentrations in the atmosphere and seasonal snow
Hydrothermal synthesis and crystal structure of catena -poly (diaqua-bis (μ 2 -4-((pyridin-4-ylmethyl) amino) benzoato-κ 2 N:O) cobalt (II)) – 4,4′-bipyridine – water (1/2/2)
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Muon (g − 2) and flavor puzzles in the U(1) X -gauged leptoquark model
A class of stochastic Gronwall’s inequality and its application
Efficient Feature Matching and Pose-graph Initialization for SfM
Rapid Thermal Drying Synthesis of Nonthiolated Spherical Nucleic Acids with Stability Rivaling Thiolated DNA
Kompakte Mehrtorantennen für die adaptive Keulenformung
Chinese Food Network in British Columbia, 1880-1940 : A Socio-economic History of Migrant Entrepreneurship
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Development of an improved two-sphere integration technique for quantifying black carbon concentrations in the atmosphere and seasonal snow
Hydrothermal synthesis and crystal structure of catena -poly (diaqua-bis (μ 2 -4-((pyridin-4-ylmethyl) amino) benzoato-κ 2 N:O) cobalt (II)) – 4,4′-bipyridine – water (1/2/2)
Boron‐Catalyzed Polymerization of Dienyltriphenylarsonium Ylides: On the Way to Pure C5 Polymerization
Steric Hindrance Drives the Boron‐Initiated Polymerization of Dienyltriphenylarsonium Ylides to Photoluminescent C5‐Polymers
Muon (g − 2) and flavor puzzles in the U(1) X -gauged leptoquark model
A class of stochastic Gronwall’s inequality and its application