Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures

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Abstract: Microglia are specialized parenchymal-resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single-cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)-injected mice. By excluding the contribution of other immune CNS-resident and peripheral cells, we show that microglia isolated from LPS-injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease-associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Notes: EMBO reports. - 19, 11 (2018) , e46171, ISSN: 1469-221X

Classification: Medizin, Gesundheit

Event: Veröffentlichung

(where): Freiburg

(who): Universität

(when): 2019

Creator: Sousa, Carole
Golebiewska, Anna
Poovathingal, Suresh K.
Kaoma, Tony
Pires‐Afonso, Yolanda
Martina, Silvia
Coowar, Djalil
Azuaje, Francisco
Skupin, Alexander
Balling, Rudolf
Biber, Knut
Niclou, Simone
Michelucci, Alessandro

Contributor: Klinik für Psychiatrie und Psychotherapie. Freiburg im Breisgau

DOI: 10.15252/embr.201846171

URN: urn:nbn:de:bsz:25-freidok-1471551

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:28 AM CEST

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Associated

Sousa, Carole
Golebiewska, Anna
Poovathingal, Suresh K.
Kaoma, Tony
Pires‐Afonso, Yolanda
Martina, Silvia
Coowar, Djalil
Azuaje, Francisco
Skupin, Alexander
Balling, Rudolf
Biber, Knut
Niclou, Simone
Michelucci, Alessandro
Klinik für Psychiatrie und Psychotherapie. Freiburg im Breisgau
Universität

Time of origin

2019

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