Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

to related objects

Abstract: In vitro selected ribozymes are promising tools for site‐specific labeling of RNA. Previously known nucleic acid catalysts attached fluorescently labeled adenosine or guanosine derivatives through 2′,5′‐branched phosphodiester bonds to the RNA of interest. Herein, we report new ribozymes that use orthogonal substrates, derived from the antiviral drug tenofovir, and attach bioorthogonal functional groups, as well as affinity handles and fluorescent reporter units through a hydrolytically more stable phosphonate ester linkage. The tenofovir transferase ribozymes were identified by in vitro selection and are orthogonal to nucleotide transferase ribozymes. As genetically encodable functional RNAs, these ribozymes may be developed for potential cellular applications. The orthogonal ribozymes addressed desired target sites in large RNAs in vitro, as shown by fluorescent labeling of E. coli 16S and 23S rRNAs in total cellular RNA.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA ; volume:132 ; number:24 ; year:2020 ; pages:9421-9425 ; extent:5
Angewandte Chemie ; 132, Heft 24 (2020), 9421-9425 (gesamt 5)

Creator: Ghaem Maghami, Mohammad
Dey, Surjendu
Lenz, Ann‐Kathrin
Höbartner, Claudia

DOI: 10.1002/ange.202001300

URN: urn:nbn:de:101:1-2022053007303842944797

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:30 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Ghaem Maghami, Mohammad
Dey, Surjendu
Lenz, Ann‐Kathrin
Höbartner, Claudia

Other Objects (12)

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

In vitro selected ribozymes for RNA methylation and labeling

In vitro selected ribozymes for RNA methylation and labeling

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

In vitro selected ribozymes for RNA methylation and labeling

In vitro selected ribozymes for RNA methylation and labeling

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

Repurpsing Antiviral Drugs for Orthogonal RNA-Catalyzed Labeling

In vitro selected ribozymes for RNA methylation and labeling

In vitro selected ribozymes for RNA methylation and labeling

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme‐Catalyzed Late‐Stage Functionalization and Fluorogenic Labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Ribozyme-catalyzed late-stage functionalization and fluorogenic labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

Direct in vitro selection of trans-acting ribozymes for posttranscriptional, site-specific, and covalent fluorescent labeling of RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

RNA‐Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Artificial Evolution of Nucleic Acid Catalysts and their Use for Studying RNA

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.