Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers
Abstract: In many tissues, cell shape and orientation are controlled by a combination of internal and external biophysical cues. Anisotropic substrate topography is a ubiquitous cue that leads to cellular elongation and alignment, a process termed contact guidance whose underlying mechanisms remain incompletely understood. Additionally, whether contact guidance responses are similar in single cells and in cellular monolayers is unknown. Here, these questions in vascular endothelial cells (ECs) that in vivo form a monolayer that lines blood vessels are addressed. Culturing single ECs on microgrooved substrates that constitute an idealized mimic of anisotropic basement membrane (BM) topography elicits a strong, groove depth‐dependent contact guidance response. This response is principally driven by persistence bias of cell protrusions in the direction of the grooves and is surprisingly insensitive to actin stress fiber disruption. Interestingly, the contact guidance response is greatly attenuated in confluent monolayers, and cell shape and alignment in that case are driven by the organization of the BM secreted by the cells, which leads to a loss of cellular interaction with the microgrooves. The present finding of distinct contact guidance mechanisms in single ECs and in EC monolayers promises to inform strategies aimed at designing topographically patterned endovascular devices.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers ; day:13 ; month:04 ; year:2023 ; extent:17
Advanced materials interfaces ; (13.04.2023) (gesamt 17)
- Creator
-
Leclech, Claire
Krishnamurthy, Apoorvaa
Muller, Laurent
Barakat, Abdul I.
- DOI
-
10.1002/admi.202202421
- URN
-
urn:nbn:de:101:1-2023041415054478470376
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
14.08.2025, 10:45 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Leclech, Claire
- Krishnamurthy, Apoorvaa
- Muller, Laurent
- Barakat, Abdul I.
Other Objects (12)
Silicon microgrooves for contact guidance of human aortic endothelial cells
Hs27 fibroblast response to contact guidance cues
Bimodal sensing of guidance cues in mechanically distinct microenvironments
Initial contact guidance during cell spreading is contractility-independent
The influence of capsaicin on the integrity of microvascular endothelial cell monolayers
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Langmuir blodgett lithography and capillary effects in topographical contact guidance
In vitro contact guidance of glioblastoma cells on metallic biomaterials
Ferromagnetic monolayers
Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers
Controlled condensation by liquid contact-induced adaptations of molecular conformations in self-assembled monolayers
Silicon microgrooves for contact guidance of human aortic endothelial cells
Hs27 fibroblast response to contact guidance cues
Bimodal sensing of guidance cues in mechanically distinct microenvironments
Initial contact guidance during cell spreading is contractility-independent
The influence of capsaicin on the integrity of microvascular endothelial cell monolayers
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Langmuir blodgett lithography and capillary effects in topographical contact guidance
In vitro contact guidance of glioblastoma cells on metallic biomaterials
Ferromagnetic monolayers
Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers
Controlled condensation by liquid contact-induced adaptations of molecular conformations in self-assembled monolayers
Silicon microgrooves for contact guidance of human aortic endothelial cells
Hs27 fibroblast response to contact guidance cues
Bimodal sensing of guidance cues in mechanically distinct microenvironments
Initial contact guidance during cell spreading is contractility-independent
The influence of capsaicin on the integrity of microvascular endothelial cell monolayers
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Physical contact between mesenchymal stem cells and endothelial precursors induces distinct signatures with relevance to tissue regeneration and engineering
Langmuir blodgett lithography and capillary effects in topographical contact guidance
In vitro contact guidance of glioblastoma cells on metallic biomaterials
Ferromagnetic monolayers
Hypoxia suppresses glucose-induced increases in collective cell migration in vascular endothelial cell monolayers