High-throughput sequencing offers new insights into 5-hydroxymethylcytosine
Abstract: Chemical modifications of DNA comprise epigenetic mechanisms that contribute to the maintenance of cellular activities and memory. Although the function of 5-methylcytosine (5-mC) has been extensively studied, little is known about the function (s) of relatively rarer and underappreciated cytosine modifications including 5-hydroxymethylcytosine (5-hmC). The discovery that ten-eleven translocation (Tet) proteins mediate conversion of 5-mC to 5-hmC, and other oxidation derivatives, sparked renewed interest to understand the biological role of 5-hmC. Studies examining total 5-hmC levels revealed the highly dynamic yet tissue-specific nature of this modification, implicating a role in epigenetic regulation and development. Intriguingly, 5-hmC levels are highest during early development and in the brain where abnormal patterns of 5-hmC have been observed in disease conditions. Thus, 5-hmC adds to the growing list of epigenetic modifications with potential utility in clinical applications and warrants further investigation. This review discusses the emerging functional roles of 5-hmC in normal and disease states, focusing primarily on insights provided by recent studies exploring the genome-wide distribution of this modification in mammals.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
High-throughput sequencing offers new insights into 5-hydroxymethylcytosine ; volume:7 ; number:3 ; year:2016 ; pages:169-178 ; extent:10
Biomolecular concepts ; 7, Heft 3 (2016), 169-178 (gesamt 10)
- Creator
-
Pang, Alina P.S.
Sugai, Christopher
Maunakea, Alika K.
- DOI
-
10.1515/bmc-2016-0011
- URN
-
urn:nbn:de:101:1-2409241622566.441000256090
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:26 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Pang, Alina P.S.
- Sugai, Christopher
- Maunakea, Alika K.
Other Objects (12)
Indices and applications in high-throughput sequencing
High-throughput sequencing: a failure mode analysis
Indices and Applications in High-Throughput Sequencing
Approximate string matching for high-throughput sequencing
Genome reassembly with high-throughput sequencing data
Exploring the transcriptome with high-throughput sequencing data
SNP discovery by high-throughput sequencing in soybean
Statistical analysis of high-throughput sequencing count data
High-throughput sequencing of black pepper root transcriptome
High-throughput sequencing and small non-coding RNAs
Microfluidics applications for high-throughput single cell sequencing
New insights into chromomere organization provided by lampbrush chromosome microdissection and high-throughput sequencing
Indices and applications in high-throughput sequencing
High-throughput sequencing: a failure mode analysis
Indices and Applications in High-Throughput Sequencing
Approximate string matching for high-throughput sequencing
Genome reassembly with high-throughput sequencing data
Exploring the transcriptome with high-throughput sequencing data
SNP discovery by high-throughput sequencing in soybean
Statistical analysis of high-throughput sequencing count data
High-throughput sequencing of black pepper root transcriptome
High-throughput sequencing and small non-coding RNAs
Microfluidics applications for high-throughput single cell sequencing
New insights into chromomere organization provided by lampbrush chromosome microdissection and high-throughput sequencing
Indices and applications in high-throughput sequencing
High-throughput sequencing: a failure mode analysis
Indices and Applications in High-Throughput Sequencing
Approximate string matching for high-throughput sequencing
Genome reassembly with high-throughput sequencing data
Exploring the transcriptome with high-throughput sequencing data
SNP discovery by high-throughput sequencing in soybean
Statistical analysis of high-throughput sequencing count data
High-throughput sequencing of black pepper root transcriptome
High-throughput sequencing and small non-coding RNAs
Microfluidics applications for high-throughput single cell sequencing