Quantitative Analysis of The High‐Yield Hydrolysis of Kelp by Laminarinase and Alginate Lyase
Abstract: Kelp is an abundant, farmable biomass‐containing laminarin and alginate as major polysaccharides, providing an excellent model substrate to study their deconstruction by simple enzyme mixtures. Our previous study showed strong reactivity of the glycoside hydrolase family 55 during hydrolysis of purified laminarin, raising the question of its reactivity with intact kelp. In this study, we determined that a combination of a single glycoside hydrolase family 55 β‐1,3‐exoglucanase with a broad‐specificity alginate lyase from the polysaccharide lyase family 18 gives efficient hydrolysis of untreated kelp to a mixture of simple sugars, that is, glucose, gentiobiose, mannitol‐end glucose, and mannuronic and guluronic acids and their soluble oligomers. Quantitative assignments from nanostructure initiator mass spectrometry (NIMS) and 2D HSQC NMR spectroscopy and analysis of the reaction time‐course are provided. The data suggest that binary combinations of enzymes targeted to the unique polysaccharide composition of marine biomass are sufficient to deconstruct kelp into soluble sugars for microbial fermentation.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Quantitative Analysis of The High‐Yield Hydrolysis of Kelp by Laminarinase and Alginate Lyase ; day:28 ; month:08 ; year:2023 ; extent:13
ChemBioChem ; (28.08.2023) (gesamt 13)
- Urheber
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Takasuka, Taichi E.
Kim, Hoon
Deng, Kai
Bianchetti, Christopher M.
Yamashita, Kaho
Beebe, Emily T.
Bergeman, Lai F.
Vander Meulen, Kirk A.
Deutsch, Samuel
Ralph, John
Adams, Paul D.
Northen, Trent R.
Fox, Brian G.
- DOI
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10.1002/cbic.202300357
- URN
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urn:nbn:de:101:1-2023082815263746573292
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 11:03 MESZ
Datenpartner
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Beteiligte
- Takasuka, Taichi E.
- Kim, Hoon
- Deng, Kai
- Bianchetti, Christopher M.
- Yamashita, Kaho
- Beebe, Emily T.
- Bergeman, Lai F.
- Vander Meulen, Kirk A.
- Deutsch, Samuel
- Ralph, John
- Adams, Paul D.
- Northen, Trent R.
- Fox, Brian G.
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