Hochschulschrift

Assembly of photosystem I in thylakoid membranes

Zusammenfassung: The light reactions of photosynthesis are carried out by a series of multiprotein complexes embedded in thylakoid membranes. Among them, photosystem I (PSI), acting as plastocyanin-ferderoxin oxidoreductase, catalyzes the final reaction. Together with light-harvesting antenna I, PSI forms a high-molecular-weight supercomplex of ~600 kDa, consisting of eighteen subunits and nearly two hundred co-factors. Assembly of the various components into a functional thylakoid membrane complex requires precise coordination, which is provided by the assembly machinery. Although this includes a small number of proteins (PSI assembly factors) that have been shown to play a role in the formation of PSI, the process as a whole, as well as the intricacy of its members, remains largely unexplored. In the present work, two approaches were used to find candidate PSI assembly factors. First, EnsembleNet was used to select proteins thought to be functionally related to known PSI assembly factors in Arabidopsis thaliana (approach I), and second, co-immunoprecipitation (Co-IP) of tagged PSI assembly factors in Nicotiana tabacum was performed (approach II). [...].

Location
Deutsche Nationalbibliothek Frankfurt am Main
Dimensions
30 cm
Extent
177 Seiten
Language
Englisch
Notes
Illustrationen
Universität Potsdam, Dissertation, 2023

Classification
Biowissenschaften, Biologie

Event
Veröffentlichung
(where)
Potsdam
(when)
[2023]
Creator

Table of contents
Rights
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Last update
11.03.2025, 12:14 PM CET

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Object type

  • Hochschulschrift

Associated

Time of origin

  • [2023]

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