Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry

Abstract: Time-domain measurement of the fluorescence lifetime in flow cytometry offers a complementary method to traditional flow cytometry, especially for the analysis of cells and biomolecules. Short measurement times due to high throughputs (cells/s) make a suited detector mandatory for a sufficient detection of the fluorescence signal and therefore the application of this method. We summarize the requirements for a suited detector for time resolved flow cytometry (TRFC) and present a new developed SPAD array detector with 100 pixels and 340 ns time between measurement windows, allowing a high-throughput of up to 60,000 cells/s. The functionality of the detector is shown with the measurement of a 22.2 ns laser pulse, making it applicable for an improved TRFC.

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• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Single-photon avalanche diode (SPAD) array detector for high-throughput fluorescence lifetime flow cytometry ; volume:9 ; number:1 ; year:2023 ; pages:363-366 ; extent:4 ; Current directions in biomedical engineering ; 9, Heft 1 (2023), 363-366 (gesamt 4)

Contributors, Places and Time

• Creator: Rottmann, Jonas ; Schierbaum, Nicolas ; Netaev, Alexander ; Ligges, Manuel ; Seidl, Karsten

Further information

• DOI: 10.1515/cdbme-2023-1091
• URN: urn:nbn:de:101:1-2023092214123429220286
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.