Early SARS-CoV-2 infection: platelet-neutrophil complexes and platelet function

Abstract: Background
Conflicting results have been reported on platelet activity ex vivo and responsiveness in vitro among patients with COVID-19 with or without thromboembolic complications.

Objectives
To assess platelet reactivity in patients with moderate disease at early stages of COVID-19.

Methods
We performed a prospective, descriptive analysis of 100 consecutive patients presenting with suspected SARS-CoV-2 infection at University Medical Center Freiburg during the first or second wave of the pandemic. Following polymerase chain reaction testing and compliance with study inclusion criteria, 20 SARS-CoV-2–positive and 55 SARS-CoV-2–negative patients (serving as patient controls) were enrolled. In addition, 15 healthy subjects were included. Platelet reactivity was assessed using whole-blood impedance aggregometry and flow cytometry in response to various agonists.

Results
Platelet aggregation was significantly impaired in the patients with COVID-19 compared with that in the patient controls or healthy subjects. The reduced platelet responsiveness in the patients with COVID-19 was associated with impaired activation of GPIIb/IIIa (αIIbβ3). In contrast, low expression of P-selectin at baseline and intact secretion upon stimulation in vitro suggest that no preactivation in vivo, leading to “exhausted” platelets, had occurred. The proportion of circulating platelet-neutrophil complexes was significantly higher in the patients with COVID-19 (mean ± SD, 41% ± 13%) than in the patient controls (18% ± 7%; 95% CI, 11.1-34.1; P = .0002) or healthy subjects (17% ± 4%; 95% CI, 13.8-33.8; P < .0001). An analysis of neutrophil adhesion receptors revealed upregulation of CD11b (α-subunit of αMβ2) and CD66b (CEACAM8) but not of CD162 (PSGL-1) in the patients with COVID-19.

Conclusion
Despite reduced platelet responsiveness, platelet-neutrophil complexes are increased at early stages of moderate disease. Thus, this cellular interaction may occur during COVID-19 without preceding platelet activation

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Research and practice in thrombosis and haemostasis. - 7, 1 (2023) , 100025, ISSN: 2475-0379

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2023
• Urheber: Rieder, Marina ; Baldus, Niklas ; Stallmann, Daniela ; Jeserch, Maren ; Goller, Isabella ; Wirth, Luisa ; Pollmeier, Luisa ; Hofmann, Maike ; Bode, Christoph ; Busch, Hans-Jörg ; Schmid, Bonaventura ; Gauchel, Nadine ; Scharf, Rüdiger Eberhard ; Dürschmied, Daniel ; Lother, Achim ; Krauel, Krystin

Weitere Informationen

• DOI: 10.1016/j.rpth.2022.100025
• URN: urn:nbn:de:bsz:25-freidok-2326224
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 25.03.2025, 13:51 MEZ