P75 neurotrophin receptor controls subventricular zone neural stem cell migration after stroke

Abstract: Stroke is the leading cause of adult disability. Endogenous neural stem/progenitor cells (NSPCs) originating from the subventricular zone (SVZ) contribute to the brain repair process. However, molecular mechanisms underlying CNS disease-induced SVZ NSPC-redirected migration to the lesion area are poorly understood. Here, we show that genetic depletion of the p75 neurotrophin receptor (p75NTR−/−) in mice reduced SVZ NSPC migration towards the lesion area after cortical injury and that p75NTR−/− NSPCs failed to migrate upon BDNF stimulation in vitro. Cortical injury rapidly increased p75NTR abundance in SVZ NSPCs via bone morphogenetic protein (BMP) receptor signaling. SVZ-derived p75NTR−/− NSPCs revealed an altered cytoskeletal network- and small GTPase family-related gene and protein expression. In accordance, BMP-treated non-migrating p75NTR−/− NSPCs revealed an altered morphology and α-tubulin expression compared to BMP-treated migrating wild-type NSPCs. We propose that BMP-induced p75NTR abundance in NSPCs is a regulator of SVZ NSPC migration to the lesion area via regulation of the cytoskeleton following cortical injury

Show object

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: Cell & tissue research. - 387 (2022) , 415–431, ISSN: 1432-0878

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2021
• Creator: Deshpande, Sachin S. ; Malik, Subash C. ; Conforti, Pasquale ; Lin, Jia-Di ; Chu, Yu-Hsuan ; Nath, Suvra ; Greulich, Franziska ; Dumbach, Meike-Ast ; Uhlenhaut, Nina Henriette ; Schachtrup, Christian

Further information

• DOI: 10.1007/s00441-021-03539-z
• URN: urn:nbn:de:bsz:25-freidok-2224887
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.