Diagnostic accuracy of a fully automated multiplex celiac disease antibody panel for serum and plasma

Background: An automated multiplex platform using capillary blood can promote greater throughput and more comprehensive studies in celiac disease (CD). Diagnostic accuracy should be improved using likelihood ratios for the post-test probability of ruling-in disease. Methods: The Ig_plex™ Celiac Disease Panel on the sqidlite™ automated platform measured IgA and IgG antibodies to tTG and DGP in n = 224 CD serum or plasma samples. Diagnostic accuracy metrics were applied to the combined multiplex test results for several CD populations and compared to conventional single antibody ELISA tests. Results: With multiple positive antibody results, the post-test probability for ruling-in untreated and treated CD increased to over 90%. The number of samples positive for more than one antibody also increased in untreated CD to ≥90%. Measurement of all four CD antibodies generate cut-off dependent accuracy profiles that can monitor response to treatment with the gluten-free diet (GFD). Higher positive tTG and DGP antibodies are seen more frequently in confirmed CD without (81%–94%) than with GFD treatment (44%–64%). In CD lacking biopsy confirmation, overall agreement of plasma to serum was ≥98% for all antibodies, and 100% for venous to capillary plasma. Conclusions: The Ig_plex Celiac Disease Panel increases the likelihood of confirming CD based on the post-test probability of disease results for multi-reactive markers. Specific positivity profiles and cut-off intervals can be used to monitor GFD treatment and likely disease progression. Using serum, venous and capillary plasma yield comparable and accurate results.

Objekt anzeigen

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Diagnostic accuracy of a fully automated multiplex celiac disease antibody panel for serum and plasma ; volume:57 ; number:8 ; year:2019 ; pages:1207-1217 ; extent:11 ; Clinical chemistry and laboratory medicine ; 57, Heft 8 (2019), 1207-1217 (gesamt 11)

Beteiligte, Orts- und Zeitangaben

• Urheber: Terryberry, Jeff ; Tuomi, Jani ; Perampalam, Subo ; Peloquin, Russ ; Brouwer, Eric ; Schuppan, Detlef ; Guandalini, Stefano

Weitere Informationen

• DOI: 10.1515/cclm-2019-0088
• URN: urn:nbn:de:101:1-2022053114311180725861
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.