Expression of human growth hormone gene in Pleurotus eryngii
Abstract: Recombinant human growth hormone (rhGH) has been widely used in many medical applications. Large scale production of rhGH is important for a wider application of this molecule in the field of proteomics. This investigation reports a modified Agrobacterium-mediated method for transfer and expression of human growth hormone gene in the popular edible mushroom Pleurotus eryngii. A binary vector pCAMBIA1304 containing the hGH2 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. Integration of hGH2 into mushroom genome was detected by PCR and expression was confirmed by Western blot assay. The successful expression of the human growth hormone gene in mushroom suggests that the proposed modified transformation system is probably useful for the production of transgenic mushrooms.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Expression of human growth hormone gene in Pleurotus eryngii ; volume:5 ; number:6 ; year:2010 ; pages:791-799 ; extent:9
Open life sciences ; 5, Heft 6 (2010), 791-799 (gesamt 9)
- Creator
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Kim, Seung
Sapkota, Kumar
Choi, Bong-Suk
Kim, Sung-Jun
- DOI
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10.2478/s11535-010-0071-8
- URN
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urn:nbn:de:101:1-2409201859211.677745026042
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:35 AM CEST
Data provider
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Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kim, Seung
- Sapkota, Kumar
- Choi, Bong-Suk
- Kim, Sung-Jun
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