Development of an Injectable Biphasic Hyaluronic Acid‐Based Hydrogel With Stress Relaxation Properties for Cartilage Regeneration

Abstract: Biomimetic stress‐relaxing hydrogels with reversible crosslinks attract significant attention for stem cell tissue regeneration compared with elastic hydrogels. However, stress‐relaxing hyaluronic acid (HA)‐based hydrogels fabricated using conventional technologies lack stability, biocompatibility, and mechanical tunability. Here, it is aimed to address these challenges by incorporating calcium or phosphate components into the HA backbone, which allows reversible crosslinking of HA with alginate to form interpenetrating networks, offering stability and mechanical tunability for mimicking cartilage. Diverse stress‐relaxing hydrogels (τ1/2; SR50, 60–2000 s) are successfully prepared at ≈3 kPa stiffness with self‐healing and shear‐thinning abilities, favoring hydrogel injection. In vitro cell experiments with RNA sequencing analysis demonstrate that hydrogels tune chondrogenesis in a biphasic manner (hyaline or calcified) depending on the stress‐relaxation properties and phosphate components. In vivo studies confirm the potential for biphasic chondrogenesis. These results indicate that the proposed stress‐relaxing HA‐based hydrogel with biphasic chondrogenesis (hyaline or calcified) is a promising material for cartilage regeneration.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Development of an Injectable Biphasic Hyaluronic Acid‐Based Hydrogel With Stress Relaxation Properties for Cartilage Regeneration ; day:09 ; month:04 ; year:2024 ; extent:17 ; Advanced healthcare materials ; (09.04.2024) (gesamt 17)

Beteiligte, Orts- und Zeitangaben

• Urheber: Kim, Han‐Sem ; Li, Cheng Ji ; Park, Sung‐Min ; Kim, Kyung Wook ; Mo, Ji‐Hun ; Jin, Guang‐Zhen ; Lee, Hae‐Hyoung ; Kim, Hae‐Won ; Shin, Ueon Sang ; Lee, Jung‐Hwan

Weitere Informationen

• DOI: 10.1002/adhm.202400043
• URN: urn:nbn:de:101:1-2024041014580596204261
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 11:04 MESZ