CD206+ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia

Abstract: The relationships between tissue-resident microglia and early macrophages, especially their lineage segregation outside the yolk sac, have been recently explored, providing a model in which a conversion from macrophages seeds microglia during brain development. However, spatiotemporal evidence to support such microglial seeding in situ and to explain how it occurs has not been obtained. By cell tracking via slice culture, intravital imaging, and Flash tag-mediated or genetic labeling, we find that intraventricular CD206+ macrophages, which are abundantly observed along the inner surface of the mouse cerebral wall, frequently enter the pallium at embryonic day 12. Immunofluorescence of the tracked cells show that postinfiltrative macrophages in the pallium acquire microglial properties while losing the CD206+ macrophage phenotype. We also find that intraventricular macrophages are supplied transepithelially from the roof plate. This study demonstrates that the “roof plate→ventricle→pallium” route is an essential path for microglial colonization into the embryonic mouse brain

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Cell reports. - 42, 2 (2023) , 112092, ISSN: 2211-1247

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2023
• Urheber: Hattori, Yuki ; Kato, Daisuke ; Murayama, Futoshi ; Koike, Sota ; Asai, Hisa ; Yamasaki, Ayato ; Naito, Yu ; Kawaguchi, Ayano ; Konishi, Hiroyuki ; Prinz, Marco ; Masuda, Takahiro ; Wake, Hiroaki ; Miyata, Takaki

Weitere Informationen

• DOI: 10.1016/j.celrep.2023.112092
• URN: urn:nbn:de:bsz:25-freidok-2340602
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:49 MESZ