Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection

Abstract: We previously reported that M. tb on its own as well as together with HIV inhibits macrophage apoptosis by upregulating the expression of Bcl2 and Inhibitor of Apoptosis (IAP). In addition, recent reports from our lab showed that stimulation of either macrophages or BMDCs results in the significant upregulation of Bcl2. In this report, we delineate the role of Bcl2 in mediating defense responses from dendritic cells (BMDCs) during mycobacterial infection. Inhibiting Bcl2 led to a significant decrease in intracellular bacterial burden in BMDCs. To further characterize the role of Bcl2 in modulating defense responses, we inhibited Bcl2 in BMDCs as well as human PBMCs to monitor their activation and functional status in response to mycobacterial infection and stimulation with M. tb antigen Rv3416. Inhibiting Bcl2 generated protective responses including increased expression of co-stimulatory molecules, oxidative burst, pro-inflammatory cytokine expression and autophagy. Finally, co-culturing human PBMCs and BMDCs with antigen-primed T cells increased their proliferation, activation and effector function. These results point towards a critical role for Bcl2 in regulating BMDCs defense responses to mycobacterial infection.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Bcl2 negatively regulates Protective Immune Responses During Mycobacterial Infection ; volume:12 ; number:1 ; year:2021 ; pages:94-109 ; extent:16 ; Biomolecular concepts ; 12, Heft 1 (2021), 94-109 (gesamt 16)

Contributors, Places and Time

• Creator: Singh, Aayushi ; Anang, Vandana ; Verma, Chaitenya ; Saraswati, Shakuntala Surender Kumar ; Rana, Ankush Kumar ; Bandyopadhyay, Upasana ; Chadha, Attinder ; Natarajan, Krishnamurthy

Further information

• DOI: 10.1515/bmc-2021-0010
• URN: urn:nbn:de:101:1-2409241639481.632661849879
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:37 AM CEST